Detailed information of TA system TA122000

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 3777584..3778289
• Replicon: chromosome
• Accession: NZ_CP038363
• Organism: Escherichia coli O157:H7 strain F7349

Toxin (Protein)

• Gene name: relE
• Uniprot ID: Q8X3N0
• Locus tag: E4U70_RS19240
• Protein ID: WP_000539519.1
• Coordinates: 3777584..3777970 (+)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: E4U70_RS19245
• Protein ID: WP_001280945.1
• Coordinates: 3777960..3778289 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E4U70_RS19220	3773588..3774214	+	627	WP_001295292.1	glutathione S-transferase GstB	-
E4U70_RS19225	3774211..3775326	-	1116	WP_000554959.1	aldose sugar dehydrogenase YliI	-
E4U70_RS19230	3775437..3775820	-	384	WP_000497137.1	biofilm formation regulator BssR	-
E4U70_RS19235	3776033..3777358	+	1326	WP_000049367.1	30S ribosomal protein S12 methylthiotransferase RimO	-
E4U70_RS19240	3777584..3777970	+	387	WP_000539519.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
E4U70_RS19245	3777960..3778289	+	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
E4U70_RS19250	3778359..3779687	-	1329	WP_000086905.1	GGDEF domain-containing protein	-
E4U70_RS19255	3779695..3782043	-	2349	WP_000950320.1	EAL domain-containing protein	-
E4U70_RS19260	3782221..3783132	-	912	WP_001236044.1	glutathione ABC transporter permease GsiD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14279.42 Da
• Isoelectric Point: 9.9296

>T122000 WP_000539519.1 NZ_CP038363:3777584-3777970 [Escherichia coli O157:H7]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGSKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T122000 NZ_CP038363:3777584-3777970 [Escherichia coli O157:H7]
ATGGGTGTGTATAAGGCAAGGCGATTCAGCCAAAGCACGAAAAAACTTGGTATTCACGATAAAGTTCTCATGGCTGCCGC
TGAAGAAGTGATGCAAGGCATATGGGAGGCTGATTTAGGTAGCGGTGTCATCAAAAAACGGTTACCCCTCCAACAAGGAA
AGAGTGGTGGAGCCAGAACAATTATCTTTTTTAAATCAGCAAATCATGTGTTTTTTTATGATGGTTGGTCCAAGAGTGGT
CTAAGCAGTAAAGGAAGCAAAGAAATCGAAGATGATGAACTGGCAGCCTACAAAAAAATGGCAAATGCATTTCTGGCCTT
TAGCAATAAGCAAATAGAGGATTTGATCGAAACTGGCTTCTTGATTGAGGTGAAAAATGAGCGATAA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT122000 WP_001280945.1 NZ_CP038363:3777960-3778289 [Escherichia coli O157:H7]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT122000 NZ_CP038363:3777960-3778289 [Escherichia coli O157:H7]
ATGAGCGATAAATTACAAATGATGCTGGAAGATGCAAAGGAACTGAATGCACTGGGCCTGATGTCTGATAGTGAGGTTGA
TGCCATTGCCGTCATGGTCAAAGCCCGTGAAGTCAGCGCCCGTATAGCCAAAGTCAAATCAATGACGGGTGAAGAGATCA
AAGCCATCCGCACCCGATACGGCATGTCACAATCCGTGCTGGCACATACAATGGGAATGTCAAAAGAAAGTGTCTCGAAA
TGGGAACGTAATGAAATAAAACCGAGCGGCCCGGCACTACGTATTTTGAATACATTGGCAATAAAAGGGCCGGAAGTTTT
TGCAAAGTAA