Detailed information of TA system TA121998

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3667053..3667851
• Replicon: chromosome
• Accession: NZ_CP038363
• Organism: Escherichia coli O157:H7 strain F7349

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A0V7WHV8
• Locus tag: E4U70_RS18800
• Protein ID: WP_001506713.1
• Coordinates: 3667053..3667430 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A093DMQ4
• Locus tag: E4U70_RS18805
• Protein ID: WP_001280952.1
• Coordinates: 3667477..3667851 (-)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E4U70_RS18770	3663734..3664438	+	705	WP_001241680.1	leucyl/phenylalanyl-tRNA--protein transferase	-
E4U70_RS18775	3664723..3664941	+	219	WP_001040187.1	translation initiation factor IF-1	-
E4U70_RS18785	3665432..3666274	-	843	WP_001688445.1	DUF4942 domain-containing protein	-
E4U70_RS18790	3666359..3666556	-	198	WP_000839282.1	DUF957 domain-containing protein	-
E4U70_RS18795	3666568..3667056	-	489	WP_001470575.1	hypothetical protein	-
E4U70_RS18800	3667053..3667430	-	378	WP_001506713.1	TA system toxin CbtA family protein	Toxin
E4U70_RS18805	3667477..3667851	-	375	WP_001280952.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
E4U70_RS18810	3668014..3668235	-	222	WP_001220316.1	DUF987 domain-containing protein	-
E4U70_RS18815	3668304..3668780	-	477	WP_001186714.1	RadC family protein	-
E4U70_RS18820	3668796..3669281	-	486	WP_001470565.1	antirestriction protein	-
E4U70_RS18825	3669373..3670191	-	819	WP_001506712.1	DUF945 domain-containing protein	-
E4U70_RS18830	3670531..3671601	-	1071	WP_000102643.1	patatin-like phospholipase family protein	-
E4U70_RS18835	3671598..3672503	-	906	WP_021497799.1	yjcZ-like family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-118)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14178.26 Da
• Isoelectric Point: 7.8045

>T121998 WP_001506713.1 NZ_CP038363:c3667430-3667053 [Escherichia coli O157:H7]
MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYITVNNITLGKHPEAKR

Nucleotide

• Length: 378 bp

>T121998 NZ_CP038363:c3667430-3667053 [Escherichia coli O157:H7]
ATGAAAACATTATCCGACACACATGTACGGGAGGTATCTCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAT
CCGACTGCTGGACCAGCATTATGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGTATTTCACTGTGTGATGCGGTGAACTTTCTCGTTGAAAAATACGCCCTGGTACGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCCGGGCAACAGGCCTGATGACCCGCGA
CAATTACATAACGGTAAATAACATTACCCTGGGGAAGCATCCGGAGGCAAAACGATGA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 13719.50 Da
• Isoelectric Point: 6.6249

>AT121998 WP_001280952.1 NZ_CP038363:c3667851-3667477 [Escherichia coli O157:H7]
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRNQHTVTLYAKGLTCEADTLGSCGYVYLAVYPAQAPATTS

Nucleotide

• Length: 375 bp

>AT121998 NZ_CP038363:c3667851-3667477 [Escherichia coli O157:H7]
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGTGCCCGTCTGGTGCAGGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAGCTCATGCTCACCAGCGGT
GAACTGAATCCCCGAAATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGGCACAGGCACCCGCAACCACCTCATAA

Structures

Toxin

• AlphaFold DB: A0A0V7WHV8

Antitoxin

• AlphaFold DB: A0A093DMQ4