Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 359321..359952 Replicon chromosome
Accession NZ_CP038363
Organism Escherichia coli O157:H7 strain F7349

Toxin (Protein)


Gene name hicA Uniprot ID Q8X5S6
Locus tag E4U70_RS01650 Protein ID WP_001259386.1
Coordinates 359321..359596 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag E4U70_RS01655 Protein ID WP_000593555.1
Coordinates 359593..359952 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E4U70_RS01635 354325..355392 + 1068 WP_000361458.1 HlyD family secretion protein -
E4U70_RS01640 355389..358124 + 2736 WP_000149107.1 ribosome-associated ATPase/putative transporter RbbA -
E4U70_RS01645 358124..359248 + 1125 WP_001301659.1 ABC transporter permease -
E4U70_RS01650 359321..359596 + 276 WP_001259386.1 type II toxin-antitoxin system HicA family toxin Toxin
E4U70_RS01655 359593..359952 + 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
E4U70_RS01660 360002..360862 - 861 WP_001131788.1 class II aldolase -
E4U70_RS01665 360894..361163 - 270 WP_000933060.1 HPr family phosphocarrier protein -
E4U70_RS01670 361153..362661 - 1509 WP_001301773.1 carbohydrate kinase -
E4U70_RS01675 362654..364012 - 1359 WP_001302220.1 PTS galactitol transporter subunit IIC -
E4U70_RS01680 364089..364370 - 282 WP_000084025.1 PTS sugar transporter subunit IIB -
E4U70_RS01685 364367..364840 - 474 WP_001161553.1 PTS sugar transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 350465..359952 9487


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10165.92 Da        Isoelectric Point: 10.7076

>T121974 WP_001259386.1 NZ_CP038363:359321-359596 [Escherichia coli O157:H7]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKLILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T121974 NZ_CP038363:359321-359596 [Escherichia coli O157:H7]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGC
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT121974 WP_000593555.1 NZ_CP038363:359593-359952 [Escherichia coli O157:H7]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT121974 NZ_CP038363:359593-359952 [Escherichia coli O157:H7]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1I9WXY7

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References