Detailed information of TA system TA121972

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsxAB/Xre(antitoxin)
• Location: 50193..50694
• Replicon: plasmid pF7386-2
• Accession: NZ_CP038361
• Organism: Escherichia coli O157:H7 strain F7386

Toxin (Protein)

• Gene name: TsxA
• Uniprot ID: Q9F571
• Locus tag: E4U67_RS26965
• Protein ID: WP_000702246.1
• Coordinates: 50434..50694 (-)
• Length: 87 a.a.

Antitoxin (Protein)

• Gene name: TsxB
• Uniprot ID: -
• Locus tag: E4U67_RS26960
• Protein ID: WP_001132407.1
• Coordinates: 50193..50444 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E4U67_RS26920	45388..46089	+	702	WP_000280995.1	hypothetical protein	-
E4U67_RS26925	46136..46735	+	600	WP_000091422.1	hypothetical protein	-
E4U67_RS26930	46755..47081	+	327	WP_000206697.1	hypothetical protein	-
E4U67_RS26935	47212..47649	-	438	WP_000466318.1	type II toxin-antitoxin system HicB family antitoxin	-
E4U67_RS26940	47674..47856	-	183	WP_000833470.1	type II toxin-antitoxin system HicA family toxin	-
E4U67_RS26945	48390..48572	+	183	WP_001481829.1	hypothetical protein	-
E4U67_RS26950	48679..48945	-	267	WP_000681608.1	type II toxin-antitoxin system HicB family antitoxin	-
E4U67_RS26955	49365..49643	-	279	WP_000545937.1	hypothetical protein	-
E4U67_RS26960	50193..50444	-	252	WP_001132407.1	transcriptional regulator	Antitoxin
E4U67_RS26965	50434..50694	-	261	WP_000702246.1	hypothetical protein	Toxin
E4U67_RS26970	50745..50969	-	225	WP_000814384.1	hypothetical protein	-
E4U67_RS26975	51067..51276	-	210	WP_000866037.1	hypothetical protein	-
E4U67_RS26980	51318..51641	-	324	WP_000427402.1	hypothetical protein	-
E4U67_RS26985	52222..52551	-	330	WP_000855118.1	hypothetical protein	-
E4U67_RS26990	53149..53814	+	666	WP_000532148.1	P-loop NTPase	-
E4U67_RS26995	54007..54177	+	171	WP_000186151.1	hypothetical protein	-
E4U67_RS27000	54195..54791	-	597	WP_000125165.1	hypothetical protein	-
E4U67_RS27005	54941..55102	-	162	WP_001366886.1	type I toxin-antitoxin system Hok family toxin	-
E4U67_RS27010	55295..55456	-	162	WP_001128311.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..58000	58000

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (8-37)

Sequences

Toxin

Protein

• Length: 87 a.a.
• Molecular weight: 9891.42 Da
• Isoelectric Point: 10.0665

>T121972 WP_000702246.1 NZ_CP038361:c50694-50434 [Escherichia coli O157:H7]
MKIRKGDRQYYLNKEGDTFHLVKRVKTFSKSATLGKTKATVKTVADLVFHEKAFDTIDFASDGLRENDKEIIFMMIQEMS
EGKNAK

Nucleotide

• Length: 261 bp

>T121972 NZ_CP038361:c50694-50434 [Escherichia coli O157:H7]
ATGAAGATAAGAAAAGGTGACAGACAATATTATCTGAATAAAGAAGGTGATACATTTCATCTGGTGAAGCGAGTAAAAAC
TTTTTCTAAATCTGCGACTTTAGGAAAAACGAAAGCAACGGTGAAAACTGTTGCCGATCTTGTTTTTCATGAGAAGGCAT
TCGACACTATCGACTTTGCCAGCGACGGATTAAGAGAGAATGATAAAGAAATTATTTTTATGATGATTCAGGAAATGAGT
GAGGGAAAAAATGCCAAATGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9404.77 Da
• Isoelectric Point: 9.8120

>AT121972 WP_001132407.1 NZ_CP038361:c50444-50193 [Escherichia coli O157:H7]
MPNENTPENIKRLRQKIGLTQKECAEIFSMSPRTWRRKEEPVGTASGTALTPVEFKFLLLLAGEHPDYVLCKRNKKNSDS
GSN

Nucleotide

• Length: 252 bp

>AT121972 NZ_CP038361:c50444-50193 [Escherichia coli O157:H7]
ATGCCAAATGAAAACACACCAGAAAACATTAAACGTCTGCGGCAAAAAATTGGCCTGACACAGAAAGAATGTGCCGAAAT
ATTTAGCATGAGTCCGCGTACCTGGCGTCGCAAAGAAGAACCTGTCGGAACTGCAAGCGGTACAGCACTTACACCAGTAG
AGTTTAAATTTCTTCTGCTTTTAGCTGGAGAACACCCTGATTATGTACTTTGTAAAAGGAATAAAAAGAATTCTGATTCT
GGAAGTAATTAA

Structures

Toxin

• AlphaFold DB: A0A3E1XBA7