Detailed information of TA system TA121970

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): chpSB/PRK09812-ChpS
• Location: 4845934..4846529
• Replicon: chromosome
• Accession: NZ_CP038360
• Organism: Escherichia coli O157:H7 strain F7386

Toxin (Protein)

• Gene name: chpB
• Uniprot ID: U9Y4M4
• Locus tag: E4U67_RS24010
• Protein ID: WP_000239579.1
• Coordinates: 4845934..4846284 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: chpS
• Uniprot ID: Q8XCF3
• Locus tag: E4U67_RS24015
• Protein ID: WP_001223210.1
• Coordinates: 4846278..4846529 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E4U67_RS23990	4841380..4842402	-	1023	WP_001301928.1	ABC transporter permease	-
E4U67_RS23995	4842416..4843918	-	1503	Protein_4697	sugar ABC transporter ATP-binding protein	-
E4U67_RS24000	4844058..4845014	-	957	WP_000265942.1	galactofuranose ABC transporter substrate-binding protein YtfQ	-
E4U67_RS24005	4845324..4845854	+	531	WP_000055075.1	inorganic diphosphatase	-
E4U67_RS24010	4845934..4846284	-	351	WP_000239579.1	endoribonuclease toxin ChpB	Toxin
E4U67_RS24015	4846278..4846529	-	252	WP_001223210.1	type II toxin-antitoxin system antitoxin ChpS	Antitoxin
E4U67_RS24020	4846741..4847082	-	342	WP_001219160.1	gamma-glutamylcyclotransferase	-
E4U67_RS24025	4847085..4850864	-	3780	WP_171877661.1	autotransporter assembly complex protein TamB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-114)

Antitoxin

• SpoVT-AbrB domain (6-49)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12511.45 Da
• Isoelectric Point: 5.6219

>T121970 WP_000239579.1 NZ_CP038360:c4846284-4845934 [Escherichia coli O157:H7]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAADEVVEEALLRLQAVVE

Nucleotide

• Length: 351 bp

>T121970 NZ_CP038360:c4846284-4845934 [Escherichia coli O157:H7]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GTGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTT
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9299.64 Da
• Isoelectric Point: 4.4201

>AT121970 WP_001223210.1 NZ_CP038360:c4846529-4846278 [Escherichia coli O157:H7]
MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEVQVSNNQLILTPISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGD
EIW

Nucleotide

• Length: 252 bp

>AT121970 NZ_CP038360:c4846529-4846278 [Escherichia coli O157:H7]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAAGTGCAGGTGAGCAACAACCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Structures

Toxin

• AlphaFold DB: A0A0E0Y8A8