Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2604372..2605010 Replicon chromosome
Accession NZ_CP038357
Organism Escherichia coli O157:H7 strain F7508

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag E4U80_RS13005 Protein ID WP_000813794.1
Coordinates 2604372..2604548 (+) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag E4U80_RS13010 Protein ID WP_001270286.1
Coordinates 2604594..2605010 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E4U80_RS12985 2599994..2601205 - 1212 WP_071525382.1 BenE family transporter YdcO -
E4U80_RS12990 2601258..2601794 + 537 WP_000429145.1 DNA-binding transcriptional regulator SutR -
E4U80_RS12995 2601867..2603828 + 1962 WP_001303492.1 23S rRNA 5-hydroxycytidine C2501 synthase -
E4U80_RS13000 2603920..2604150 - 231 WP_000494244.1 YncJ family protein -
E4U80_RS13005 2604372..2604548 + 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
E4U80_RS13010 2604594..2605010 + 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
E4U80_RS13015 2605089..2606495 + 1407 WP_000760654.1 PLP-dependent aminotransferase family protein -
E4U80_RS13020 2606740..2607885 + 1146 WP_000047432.1 ABC transporter substrate-binding protein -
E4U80_RS13025 2607903..2608916 + 1014 WP_000220402.1 ABC transporter ATP-binding protein -
E4U80_RS13030 2608917..2609858 + 942 WP_001251320.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T121922 WP_000813794.1 NZ_CP038357:2604372-2604548 [Escherichia coli O157:H7]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T121922 NZ_CP038357:2604372-2604548 [Escherichia coli O157:H7]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT121922 WP_001270286.1 NZ_CP038357:2604594-2605010 [Escherichia coli O157:H7]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT121922 NZ_CP038357:2604594-2605010 [Escherichia coli O157:H7]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAAGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCAGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References