Detailed information of TA system TA121921

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): paaR-paaA-parE/-
• Location: 2511598..2512069
• Replicon: chromosome
• Accession: NZ_CP038357
• Organism: Escherichia coli O157:H7 strain F7508

Toxin (Protein)

• Gene name: parE_1
• Uniprot ID: A0A0D7C2L1
• Locus tag: E4U80_RS12520
• Protein ID: WP_001303511.1
• Coordinates: 2511598..2511876 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: paaA
• Uniprot ID: Q8XAD5
• Locus tag: E4U80_RS12525
• Protein ID: WP_001302048.1
• Coordinates: 2511878..2512069 (-)
• Length: 64 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E4U80_RS12485	2506823..2507074	-	252	WP_000005552.1	excisionase family protein	-
E4U80_RS12490	2507147..2509618	-	2472	WP_000048458.1	exonuclease	-
E4U80_RS12495	2509711..2509902	-	192	WP_001090200.1	DUF1482 family protein	-
E4U80_RS12500	2509899..2510087	-	189	WP_000449192.1	cell division inhibition protein DicB	-
E4U80_RS12505	2510488..2510652	+	165	WP_001331716.1	hypothetical protein	-
E4U80_RS12510	2510656..2510874	-	219	WP_001171930.1	hypothetical protein	-
E4U80_RS12515	2510946..2511245	-	300	WP_001240334.1	hypothetical protein	-
E4U80_RS12520	2511598..2511876	-	279	WP_001303511.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
E4U80_RS12525	2511878..2512069	-	192	WP_001302048.1	hypothetical protein	Antitoxin
E4U80_RS12530	2512090..2512461	-	372	WP_001169686.1	hypothetical protein	-
E4U80_RS12535	2512559..2512861	+	303	WP_000172738.1	transcriptional regulator	-
E4U80_RS12540	2512858..2513283	+	426	WP_000693943.1	Rha family transcriptional regulator	-
E4U80_RS12545	2513306..2514268	+	963	WP_000095669.1	helix-turn-helix domain-containing protein	-
E4U80_RS12550	2514275..2515015	+	741	WP_000788938.1	ATP-binding protein	-
E4U80_RS12555	2515041..2515810	+	770	Protein_2457	DUF1627 domain-containing protein	-
E4U80_RS12560	2515826..2516221	+	396	WP_001118159.1	DUF977 family protein	-
E4U80_RS12565	2516278..2516862	+	585	WP_000206793.1	DUF551 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2503311..2549337	46026

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10661.25 Da
• Isoelectric Point: 5.5647

>T121921 WP_001303511.1 NZ_CP038357:c2511876-2511598 [Escherichia coli O157:H7]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Nucleotide

• Length: 279 bp

>T121921 NZ_CP038357:c2511876-2511598 [Escherichia coli O157:H7]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin

Protein

• Length: 64 a.a.
• Molecular weight: 7463.47 Da
• Isoelectric Point: 8.6828

>AT121921 WP_001302048.1 NZ_CP038357:c2512069-2511878 [Escherichia coli O157:H7]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Nucleotide

• Length: 192 bp

>AT121921 NZ_CP038357:c2512069-2511878 [Escherichia coli O157:H7]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Structures

Toxin

• PDB: 5CZF
• PDB: 5CW7
• PDB: 5CZE

Antitoxin

• PDB: 5CZF
• PDB: 3ZBE
• PDB: 5CW7
• AlphaFold DB: A0A829CTP0