Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 33582..34107 Replicon plasmid pF8092B-1
Accession NZ_CP038356
Organism Escherichia coli O157:H7 str. F8092B

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag E4U83_RS27615 Protein ID WP_001159868.1
Coordinates 33802..34107 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag E4U83_RS27610 Protein ID WP_000813634.1
Coordinates 33582..33800 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E4U83_RS27580 28829..29095 + 267 WP_077696845.1 serine/threonine protein kinase -
E4U83_RS27585 29216..29956 + 741 WP_001066920.1 tyrosine-type recombinase/integrase -
E4U83_RS27590 30241..31218 - 978 WP_000361615.1 RepB family plasmid replication initiator protein -
E4U83_RS27595 31626..31826 - 201 WP_000708307.1 hypothetical protein -
E4U83_RS27600 31823..32443 - 621 WP_001248529.1 hypothetical protein -
E4U83_RS27605 32440..33123 - 684 WP_010891291.1 DNA-binding protein -
E4U83_RS27610 33582..33800 + 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
E4U83_RS27615 33802..34107 + 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
E4U83_RS27620 34108..34914 + 807 WP_000016989.1 site-specific integrase -
E4U83_RS27630 36926..37681 + 756 WP_000852148.1 replication initiation protein RepE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - stcE / exeE / exeG / hlyC / hlyA / hlyB / hlyD / toxB / espP 1..98062 98062


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T121903 WP_001159868.1 NZ_CP038356:33802-34107 [Escherichia coli O157:H7 str. F8092B]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T121903 NZ_CP038356:33802-34107 [Escherichia coli O157:H7 str. F8092B]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT121903 WP_000813634.1 NZ_CP038356:33582-33800 [Escherichia coli O157:H7 str. F8092B]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT121903 NZ_CP038356:33582-33800 [Escherichia coli O157:H7 str. F8092B]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References