Detailed information of TA system TA121893

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): paaR-paaA-parE/-
• Location: 3533720..3534198
• Replicon: chromosome
• Accession: NZ_CP038355
• Organism: Escherichia coli O157:H7 str. F8092B

Toxin (Protein)

• Gene name: parE_1
• Uniprot ID: A0A891SFN9
• Locus tag: E4U83_RS18135
• Protein ID: WP_001303876.1
• Coordinates: 3533911..3534198 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: paaA
• Uniprot ID: Q8XD67
• Locus tag: E4U83_RS18130
• Protein ID: WP_000536233.1
• Coordinates: 3533720..3533911 (+)
• Length: 64 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E4U83_RS18095	3529124..3529894	-	771	WP_000450992.1	DUF1627 domain-containing protein	-
E4U83_RS18100	3529916..3530662	-	747	WP_000788751.1	ATP-binding protein	-
E4U83_RS18105	3530669..3531760	-	1092	WP_001205823.1	hypothetical protein	-
E4U83_RS18110	3531839..3532294	+	456	WP_000273724.1	hypothetical protein	-
E4U83_RS18115	3532501..3532926	-	426	WP_000693855.1	Rha family transcriptional regulator	-
E4U83_RS18120	3532910..3533182	-	273	WP_000887453.1	helix-turn-helix domain-containing protein	-
E4U83_RS18125	3533291..3533692	+	402	WP_000986592.1	helix-turn-helix domain-containing protein	-
E4U83_RS18130	3533720..3533911	+	192	WP_000536233.1	hypothetical protein	Antitoxin
E4U83_RS18135	3533911..3534198	+	288	WP_001303876.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
E4U83_RS18140	3534476..3534631	+	156	WP_000379575.1	DUF1391 family protein	-
E4U83_RS18145	3534773..3535162	+	390	WP_000394511.1	hypothetical protein	-
E4U83_RS18150	3535349..3535534	-	186	WP_001133046.1	hypothetical protein	-
E4U83_RS18155	3536108..3536296	+	189	WP_000413705.1	cell division inhibition protein DicB	-
E4U83_RS18160	3536293..3536484	+	192	WP_001098307.1	DUF1482 family protein	-
E4U83_RS18165	3536578..3539049	+	2472	WP_000034474.1	exonuclease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3492299..3541423	49124

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 10978.84 Da
• Isoelectric Point: 10.1360

>T121893 WP_001303876.1 NZ_CP038355:3533911-3534198 [Escherichia coli O157:H7 str. F8092B]
MLPILWLPSARDDLRQIITYIAKENPPAARRLKIRIETSVLPLSEHPYLYPPSERVSGLREIVTHPNYIILYRVAASSIE
IVSVTHSRRQFPFSI

Nucleotide

• Length: 288 bp

>T121893 NZ_CP038355:3533911-3534198 [Escherichia coli O157:H7 str. F8092B]
ATGTTACCCATTTTATGGCTACCGTCTGCTCGCGATGATTTGCGCCAGATCATAACTTACATCGCCAAGGAGAACCCACC
GGCAGCACGTAGACTAAAAATACGCATTGAAACATCGGTATTACCTCTATCTGAGCATCCGTACTTATATCCACCAAGCG
AACGGGTTTCTGGATTGAGAGAGATCGTGACCCACCCTAACTACATAATCCTGTACAGAGTAGCTGCTTCAAGCATTGAG
ATTGTAAGCGTGACACATTCTCGGCGACAATTTCCCTTCTCTATCTGA

Antitoxin

Protein

• Length: 64 a.a.
• Molecular weight: 7025.80 Da
• Isoelectric Point: 4.0270

>AT121893 WP_000536233.1 NZ_CP038355:3533720-3533911 [Escherichia coli O157:H7 str. F8092B]
MGTALSPIVSEFETTEQENSYNEWLRTKVTSSLADTRPAIPHDEVMAEMENLIAQIAVTNKSE

Nucleotide

• Length: 192 bp

>AT121893 NZ_CP038355:3533720-3533911 [Escherichia coli O157:H7 str. F8092B]
ATGGGTACAGCCCTTTCTCCGATAGTTTCAGAATTCGAAACTACCGAACAAGAAAACAGTTACAACGAATGGTTGCGCAC
TAAAGTAACGTCAAGCCTTGCAGACACTCGCCCCGCAATTCCACATGACGAGGTAATGGCTGAAATGGAAAATCTTATTG
CTCAAATTGCTGTAACTAACAAGAGCGAGTAA