Detailed information of TA system TA121884

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): paaR-paaA-parE/-
• Location: 2540047..2540518
• Replicon: chromosome
• Accession: NZ_CP038355
• Organism: Escherichia coli O157:H7 str. F8092B

Toxin (Protein)

• Gene name: parE_1
• Uniprot ID: A0A0D7C2L1
• Locus tag: E4U83_RS12720
• Protein ID: WP_001303511.1
• Coordinates: 2540047..2540325 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: paaA
• Uniprot ID: Q8XAD5
• Locus tag: E4U83_RS12725
• Protein ID: WP_001302048.1
• Coordinates: 2540327..2540518 (-)
• Length: 64 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E4U83_RS12680	2535053..2535237	-	185	Protein_2477	DUF3596 domain-containing protein	-
E4U83_RS12685	2535272..2535523	-	252	WP_000005552.1	excisionase family protein	-
E4U83_RS12690	2535596..2538067	-	2472	WP_000048458.1	exonuclease	-
E4U83_RS12695	2538160..2538351	-	192	WP_001090200.1	DUF1482 family protein	-
E4U83_RS12700	2538348..2538536	-	189	WP_000449192.1	cell division inhibition protein DicB	-
E4U83_RS12705	2538937..2539101	+	165	WP_001331716.1	hypothetical protein	-
E4U83_RS12710	2539105..2539323	-	219	WP_001171930.1	hypothetical protein	-
E4U83_RS12715	2539395..2539694	-	300	WP_001240334.1	hypothetical protein	-
E4U83_RS12720	2540047..2540325	-	279	WP_001303511.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
E4U83_RS12725	2540327..2540518	-	192	WP_001302048.1	hypothetical protein	Antitoxin
E4U83_RS12730	2540539..2540910	-	372	WP_001169686.1	hypothetical protein	-
E4U83_RS12735	2541008..2541310	+	303	WP_000172738.1	transcriptional regulator	-
E4U83_RS12740	2541307..2541732	+	426	WP_000693943.1	Rha family transcriptional regulator	-
E4U83_RS12745	2541755..2542717	+	963	WP_077696847.1	helix-turn-helix domain-containing protein	-
E4U83_RS12750	2542724..2543464	+	741	WP_000788936.1	ATP-binding protein	-
E4U83_RS12755	2543490..2544259	+	770	Protein_2492	DUF1627 domain-containing protein	-
E4U83_RS12760	2544275..2544670	+	396	WP_001118159.1	DUF977 family protein	-
E4U83_RS12765	2544727..2545311	+	585	WP_000206793.1	DUF551 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2531760..2586581	54821

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10661.25 Da
• Isoelectric Point: 5.5647

>T121884 WP_001303511.1 NZ_CP038355:c2540325-2540047 [Escherichia coli O157:H7 str. F8092B]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Nucleotide

• Length: 279 bp

>T121884 NZ_CP038355:c2540325-2540047 [Escherichia coli O157:H7 str. F8092B]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin

Protein

• Length: 64 a.a.
• Molecular weight: 7463.47 Da
• Isoelectric Point: 8.6828

>AT121884 WP_001302048.1 NZ_CP038355:c2540518-2540327 [Escherichia coli O157:H7 str. F8092B]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Nucleotide

• Length: 192 bp

>AT121884 NZ_CP038355:c2540518-2540327 [Escherichia coli O157:H7 str. F8092B]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Structures

Toxin

• PDB: 5CZF
• PDB: 5CW7
• PDB: 5CZE

Antitoxin

• PDB: 5CZF
• PDB: 3ZBE
• PDB: 5CW7
• AlphaFold DB: A0A829CTP0