Detailed information of TA system TA121849

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): paaR-paaA-parE/-
• Location: 2621323..2621794
• Replicon: chromosome
• Accession: NZ_CP038353
• Organism: Escherichia coli O157:H7 strain F8797

Toxin (Protein)

• Gene name: parE_1
• Uniprot ID: A0A0D7C2L1
• Locus tag: E4U81_RS13295
• Protein ID: WP_001303511.1
• Coordinates: 2621323..2621601 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: paaA
• Uniprot ID: Q8XAD5
• Locus tag: E4U81_RS13300
• Protein ID: WP_001302048.1
• Coordinates: 2621603..2621794 (-)
• Length: 64 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E4U81_RS13265	2618258..2619343	-	1086	Protein_2591	exonuclease VIII	-
E4U81_RS13270	2619436..2619627	-	192	WP_001090200.1	DUF1482 family protein	-
E4U81_RS13275	2619624..2619812	-	189	WP_000449192.1	cell division inhibition protein DicB	-
E4U81_RS13280	2620213..2620377	+	165	WP_001331716.1	hypothetical protein	-
E4U81_RS13285	2620381..2620599	-	219	WP_001171930.1	hypothetical protein	-
E4U81_RS13290	2620671..2620970	-	300	WP_001240334.1	hypothetical protein	-
E4U81_RS13295	2621323..2621601	-	279	WP_001303511.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
E4U81_RS13300	2621603..2621794	-	192	WP_001302048.1	hypothetical protein	Antitoxin
E4U81_RS13305	2621815..2622186	-	372	WP_001169686.1	hypothetical protein	-
E4U81_RS13310	2622284..2622586	+	303	WP_000172738.1	transcriptional regulator	-
E4U81_RS13315	2622583..2623008	+	426	WP_000693943.1	Rha family transcriptional regulator	-
E4U81_RS13320	2623031..2623993	+	963	WP_000095669.1	helix-turn-helix domain-containing protein	-
E4U81_RS13325	2624000..2624740	+	741	WP_000788938.1	ATP-binding protein	-
E4U81_RS13330	2624766..2625535	+	770	Protein_2604	DUF1627 domain-containing protein	-
E4U81_RS13335	2625551..2625946	+	396	WP_001118159.1	DUF977 family protein	-
E4U81_RS13340	2626003..2626587	+	585	WP_000206793.1	DUF551 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	nleG7'	2605130..2704196	99066

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10661.25 Da
• Isoelectric Point: 5.5647

>T121849 WP_001303511.1 NZ_CP038353:c2621601-2621323 [Escherichia coli O157:H7]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Nucleotide

• Length: 279 bp

>T121849 NZ_CP038353:c2621601-2621323 [Escherichia coli O157:H7]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin

Protein

• Length: 64 a.a.
• Molecular weight: 7463.47 Da
• Isoelectric Point: 8.6828

>AT121849 WP_001302048.1 NZ_CP038353:c2621794-2621603 [Escherichia coli O157:H7]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Nucleotide

• Length: 192 bp

>AT121849 NZ_CP038353:c2621794-2621603 [Escherichia coli O157:H7]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Structures

Toxin

• PDB: 5CZF
• PDB: 5CW7
• PDB: 5CZE

Antitoxin

• PDB: 5CZF
• PDB: 3ZBE
• PDB: 5CW7
• AlphaFold DB: A0A829CTP0