Detailed information of TA system TA121814

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): paaR-paaA-parE/-
• Location: 2590495..2590966
• Replicon: chromosome
• Accession: NZ_CP038351
• Organism: Escherichia coli O157:H7 strain F8798

Toxin (Protein)

• Gene name: parE_1
• Uniprot ID: A0A0D7C2L1
• Locus tag: E4U69_RS13105
• Protein ID: WP_001303511.1
• Coordinates: 2590495..2590773 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: paaA
• Uniprot ID: Q8XAD5
• Locus tag: E4U69_RS13110
• Protein ID: WP_001302048.1
• Coordinates: 2590775..2590966 (-)
• Length: 64 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E4U69_RS13065	2585501..2585685	-	185	Protein_2554	DUF3596 domain-containing protein	-
E4U69_RS13070	2585720..2585971	-	252	WP_000005552.1	excisionase family protein	-
E4U69_RS13075	2586044..2588515	-	2472	WP_000048458.1	exonuclease	-
E4U69_RS13080	2588608..2588799	-	192	WP_001090200.1	DUF1482 family protein	-
E4U69_RS13085	2588796..2588984	-	189	WP_000449192.1	cell division inhibition protein DicB	-
E4U69_RS13090	2589385..2589549	+	165	WP_001331716.1	hypothetical protein	-
E4U69_RS13095	2589553..2589771	-	219	WP_001171930.1	hypothetical protein	-
E4U69_RS13100	2589843..2590142	-	300	WP_001240334.1	hypothetical protein	-
E4U69_RS13105	2590495..2590773	-	279	WP_001303511.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
E4U69_RS13110	2590775..2590966	-	192	WP_001302048.1	hypothetical protein	Antitoxin
E4U69_RS13115	2590987..2591358	-	372	WP_001169686.1	hypothetical protein	-
E4U69_RS13120	2591456..2591758	+	303	WP_000172738.1	transcriptional regulator	-
E4U69_RS13125	2591755..2592180	+	426	WP_000693943.1	Rha family transcriptional regulator	-
E4U69_RS13130	2592203..2593165	+	963	WP_000095669.1	helix-turn-helix domain-containing protein	-
E4U69_RS13135	2593172..2593912	+	741	WP_000788938.1	ATP-binding protein	-
E4U69_RS13140	2593938..2594707	+	770	Protein_2569	DUF1627 domain-containing protein	-
E4U69_RS13145	2594723..2595118	+	396	WP_001118159.1	DUF977 family protein	-
E4U69_RS13150	2595175..2595759	+	585	WP_000206793.1	DUF551 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2582208..2688203	105995

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10661.25 Da
• Isoelectric Point: 5.5647

>T121814 WP_001303511.1 NZ_CP038351:c2590773-2590495 [Escherichia coli O157:H7]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Nucleotide

• Length: 279 bp

>T121814 NZ_CP038351:c2590773-2590495 [Escherichia coli O157:H7]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin

Protein

• Length: 64 a.a.
• Molecular weight: 7463.47 Da
• Isoelectric Point: 8.6828

>AT121814 WP_001302048.1 NZ_CP038351:c2590966-2590775 [Escherichia coli O157:H7]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Nucleotide

• Length: 192 bp

>AT121814 NZ_CP038351:c2590966-2590775 [Escherichia coli O157:H7]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Structures

Toxin

• PDB: 5CZF
• PDB: 5CW7
• PDB: 5CZE

Antitoxin

• PDB: 5CZF
• PDB: 3ZBE
• PDB: 5CW7
• AlphaFold DB: A0A829CTP0