Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 359368..359999 Replicon chromosome
Accession NZ_CP038351
Organism Escherichia coli O157:H7 strain F8798

Toxin (Protein)


Gene name hicA Uniprot ID Q8X5S6
Locus tag E4U69_RS01645 Protein ID WP_001259386.1
Coordinates 359368..359643 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag E4U69_RS01650 Protein ID WP_000593555.1
Coordinates 359640..359999 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E4U69_RS01630 354372..355439 + 1068 WP_000361458.1 HlyD family secretion protein -
E4U69_RS01635 355436..358171 + 2736 WP_000149107.1 ribosome-associated ATPase/putative transporter RbbA -
E4U69_RS01640 358171..359295 + 1125 WP_001301659.1 ABC transporter permease -
E4U69_RS01645 359368..359643 + 276 WP_001259386.1 type II toxin-antitoxin system HicA family toxin Toxin
E4U69_RS01650 359640..359999 + 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
E4U69_RS01655 360049..360909 - 861 WP_001131788.1 class II aldolase -
E4U69_RS01660 360941..361210 - 270 WP_000933060.1 HPr family phosphocarrier protein -
E4U69_RS01665 361200..362708 - 1509 WP_001301773.1 carbohydrate kinase -
E4U69_RS01670 362701..364059 - 1359 WP_001302220.1 PTS galactitol transporter subunit IIC -
E4U69_RS01675 364136..364417 - 282 WP_000084025.1 PTS sugar transporter subunit IIB -
E4U69_RS01680 364414..364887 - 474 WP_001161553.1 PTS sugar transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 350512..359999 9487


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10165.92 Da        Isoelectric Point: 10.7076

>T121798 WP_001259386.1 NZ_CP038351:359368-359643 [Escherichia coli O157:H7]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKLILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T121798 NZ_CP038351:359368-359643 [Escherichia coli O157:H7]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGC
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT121798 WP_000593555.1 NZ_CP038351:359640-359999 [Escherichia coli O157:H7]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT121798 NZ_CP038351:359640-359999 [Escherichia coli O157:H7]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1I9WXY7

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References