Detailed information of TA system TA121720

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): chpSB/PRK09812-ChpS
• Location: 4850246..4850841
• Replicon: chromosome
• Accession: NZ_CP038344
• Organism: Escherichia coli O157:H7 strain Gim1-1

Toxin (Protein)

• Gene name: chpB
• Uniprot ID: U9Y4M4
• Locus tag: E4U63_RS23885
• Protein ID: WP_000239579.1
• Coordinates: 4850246..4850596 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: chpS
• Uniprot ID: Q8XCF3
• Locus tag: E4U63_RS23890
• Protein ID: WP_001223210.1
• Coordinates: 4850590..4850841 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E4U63_RS23865	4845692..4846714	-	1023	WP_001301928.1	ABC transporter permease	-
E4U63_RS23870	4846728..4848230	-	1503	WP_000205805.1	sugar ABC transporter ATP-binding protein	-
E4U63_RS23875	4848370..4849326	-	957	WP_000265942.1	galactofuranose ABC transporter substrate-binding protein YtfQ	-
E4U63_RS23880	4849636..4850166	+	531	WP_000055075.1	inorganic diphosphatase	-
E4U63_RS23885	4850246..4850596	-	351	WP_000239579.1	endoribonuclease toxin ChpB	Toxin
E4U63_RS23890	4850590..4850841	-	252	WP_001223210.1	type II toxin-antitoxin system antitoxin ChpS	Antitoxin
E4U63_RS23895	4851053..4851394	-	342	WP_001219160.1	gamma-glutamylcyclotransferase	-
E4U63_RS23900	4851397..4855176	-	3780	WP_000060930.1	autotransporter assembly complex protein TamB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-114)

Antitoxin

• SpoVT-AbrB domain (6-49)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12511.45 Da
• Isoelectric Point: 5.6219

>T121720 WP_000239579.1 NZ_CP038344:c4850596-4850246 [Escherichia coli O157:H7]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAADEVVEEALLRLQAVVE

Nucleotide

• Length: 351 bp

>T121720 NZ_CP038344:c4850596-4850246 [Escherichia coli O157:H7]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GTGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTT
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9299.64 Da
• Isoelectric Point: 4.4201

>AT121720 WP_001223210.1 NZ_CP038344:c4850841-4850590 [Escherichia coli O157:H7]
MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEVQVSNNQLILTPISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGD
EIW

Nucleotide

• Length: 252 bp

>AT121720 NZ_CP038344:c4850841-4850590 [Escherichia coli O157:H7]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAAGTGCAGGTGAGCAACAACCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Structures

Toxin

• AlphaFold DB: A0A0E0Y8A8