Detailed information of TA system TA121653

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsxAB/Xre(antitoxin)
• Location: 31105..31606
• Replicon: plasmid pH6437-2
• Accession: NZ_CP038340
• Organism: Escherichia coli O157:H7 strain H6437

Toxin (Protein)

• Gene name: TsxA
• Uniprot ID: A0A6X6XTU7
• Locus tag: E3164_RS27245
• Protein ID: WP_000702245.1
• Coordinates: 31105..31365 (+)
• Length: 87 a.a.

Antitoxin (Protein)

• Gene name: TsxB
• Uniprot ID: -
• Locus tag: E3164_RS27250
• Protein ID: WP_001132407.1
• Coordinates: 31355..31606 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3164_RS27200	26347..26508	+	162	WP_001128311.1	hypothetical protein	-
E3164_RS27205	26702..26863	+	162	WP_001366886.1	type I toxin-antitoxin system Hok family toxin	-
E3164_RS27210	27013..27609	+	597	WP_000125165.1	hypothetical protein	-
E3164_RS27215	27627..27797	-	171	WP_000186151.1	hypothetical protein	-
E3164_RS27220	27985..28650	-	666	WP_000532148.1	P-loop NTPase	-
E3164_RS27225	29248..29577	+	330	WP_000855121.1	hypothetical protein	-
E3164_RS27230	30158..30481	+	324	WP_000427402.1	hypothetical protein	-
E3164_RS27235	30522..30731	+	210	WP_000866037.1	hypothetical protein	-
E3164_RS27240	30829..31053	+	225	WP_000814383.1	hypothetical protein	-
E3164_RS27245	31105..31365	+	261	WP_000702245.1	hypothetical protein	Toxin
E3164_RS27250	31355..31606	+	252	WP_001132407.1	transcriptional regulator	Antitoxin
E3164_RS27255	32156..32434	+	279	WP_000545937.1	hypothetical protein	-
E3164_RS27260	32821..33003	-	183	WP_001401700.1	hypothetical protein	-
E3164_RS27265	33561..33743	+	183	WP_000833470.1	type II toxin-antitoxin system HicA family toxin	-
E3164_RS27270	33768..34205	+	438	WP_000466318.1	type II toxin-antitoxin system HicB family antitoxin	-
E3164_RS27275	34336..34656	-	321	WP_000206710.1	hypothetical protein	-
E3164_RS27280	34667..34930	-	264	WP_000224227.1	hypothetical protein	-
E3164_RS27285	34932..35384	-	453	WP_000206702.1	hypothetical protein	-
E3164_RS27290	35404..36003	-	600	WP_000091423.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..56083	56083

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (8-37)

Sequences

Toxin

Protein

• Length: 87 a.a.
• Molecular weight: 9818.23 Da
• Isoelectric Point: 9.5163

>T121653 WP_000702245.1 NZ_CP038340:31105-31365 [Escherichia coli O157:H7]
MKIRKGDRQYYLNKEGDTFHLVKRVKTFSKSATLGKTKATVKTVADLVFHEEAFDTIDFASDGLRENDKEIVSMMIQEMS
EGKNAK

Nucleotide

• Length: 261 bp

>T121653 NZ_CP038340:31105-31365 [Escherichia coli O157:H7]
ATGAAGATAAGAAAAGGTGACAGACAATATTATCTGAATAAAGAAGGTGATACGTTTCATCTGGTGAAGCGAGTAAAAAC
TTTTTCTAAGTCTGCGACTTTAGGAAAAACGAAAGCAACGGTGAAAACTGTTGCTGATCTTGTTTTTCATGAGGAGGCAT
TCGACACTATCGACTTTGCCAGCGACGGATTAAGAGAGAATGATAAAGAAATTGTTTCTATGATGATTCAGGAAATGAGT
GAGGGAAAAAATGCCAAATGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9404.77 Da
• Isoelectric Point: 9.8120

>AT121653 WP_001132407.1 NZ_CP038340:31355-31606 [Escherichia coli O157:H7]
MPNENTPENIKRLRQKIGLTQKECAEIFSMSPRTWRRKEEPVGTASGTALTPVEFKFLLLLAGEHPDYVLCKRNKKNSDS
GSN

Nucleotide

• Length: 252 bp

>AT121653 NZ_CP038340:31355-31606 [Escherichia coli O157:H7]
ATGCCAAATGAAAACACACCAGAAAACATTAAACGTCTGCGGCAAAAAATTGGCCTGACACAGAAAGAATGTGCTGAAAT
ATTCAGCATGAGTCCGCGTACCTGGCGTCGCAAAGAAGAACCTGTCGGAACTGCAAGCGGTACAGCACTTACACCAGTAG
AGTTTAAATTTCTTCTGCTTTTAGCTGGAGAACACCCTGATTATGTACTTTGTAAAAGGAATAAAAAGAATTCTGATTCT
GGAAGTAATTAA

Structures

Toxin

• AlphaFold DB: A0A6X6XTU7