Detailed information of TA system TA121636

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): paaR-paaA-parE/-
• Location: 2590128..2590599
• Replicon: chromosome
• Accession: NZ_CP038339
• Organism: Escherichia coli O157:H7 strain H6437

Toxin (Protein)

• Gene name: parE_1
• Uniprot ID: A0A0D7C2L1
• Locus tag: E3164_RS13030
• Protein ID: WP_001303511.1
• Coordinates: 2590128..2590406 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: paaA
• Uniprot ID: Q8XAD5
• Locus tag: E3164_RS13035
• Protein ID: WP_001302048.1
• Coordinates: 2590408..2590599 (-)
• Length: 64 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3164_RS12990	2585135..2585319	-	185	Protein_2539	DUF3596 domain-containing protein	-
E3164_RS12995	2585354..2585605	-	252	WP_000005552.1	excisionase family protein	-
E3164_RS13000	2585678..2588149	-	2472	WP_000048458.1	exonuclease	-
E3164_RS13005	2588242..2588433	-	192	WP_001090200.1	DUF1482 family protein	-
E3164_RS13010	2588430..2588618	-	189	WP_000449192.1	cell division inhibition protein DicB	-
E3164_RS13015	2589019..2589183	+	165	WP_001331716.1	hypothetical protein	-
E3164_RS13020	2589187..2589405	-	219	WP_001171930.1	hypothetical protein	-
E3164_RS13025	2589477..2589776	-	300	WP_001240334.1	hypothetical protein	-
E3164_RS13030	2590128..2590406	-	279	WP_001303511.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
E3164_RS13035	2590408..2590599	-	192	WP_001302048.1	hypothetical protein	Antitoxin
E3164_RS13040	2590620..2590991	-	372	WP_001169686.1	hypothetical protein	-
E3164_RS13045	2591089..2591391	+	303	WP_000172738.1	transcriptional regulator	-
E3164_RS13050	2591388..2591813	+	426	WP_000693943.1	Rha family transcriptional regulator	-
E3164_RS13055	2591836..2592798	+	963	WP_000095669.1	helix-turn-helix domain-containing protein	-
E3164_RS13060	2592805..2593545	+	741	WP_000788938.1	ATP-binding protein	-
E3164_RS13065	2593571..2594340	+	770	Protein_2554	DUF1627 domain-containing protein	-
E3164_RS13070	2594356..2594751	+	396	WP_001118159.1	DUF977 family protein	-
E3164_RS13075	2594808..2595392	+	585	WP_000206793.1	DUF551 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	nleG7'	2575249..2680004	104755

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10661.25 Da
• Isoelectric Point: 5.5647

>T121636 WP_001303511.1 NZ_CP038339:c2590406-2590128 [Escherichia coli O157:H7]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Nucleotide

• Length: 279 bp

>T121636 NZ_CP038339:c2590406-2590128 [Escherichia coli O157:H7]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin

Protein

• Length: 64 a.a.
• Molecular weight: 7463.47 Da
• Isoelectric Point: 8.6828

>AT121636 WP_001302048.1 NZ_CP038339:c2590599-2590408 [Escherichia coli O157:H7]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Nucleotide

• Length: 192 bp

>AT121636 NZ_CP038339:c2590599-2590408 [Escherichia coli O157:H7]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Structures

Toxin

• PDB: 5CZF
• PDB: 5CW7
• PDB: 5CZE

Antitoxin

• PDB: 5CZF
• PDB: 3ZBE
• PDB: 5CW7
• AlphaFold DB: A0A829CTP0