Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 78958..79583 Replicon plasmid pLSU61-1
Accession NZ_CP038338
Organism Escherichia coli O157:H7 strain LSU61

Toxin (Protein)


Gene name vapC Uniprot ID -
Locus tag E3165_RS27890 Protein ID WP_000911317.1
Coordinates 78958..79356 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID L4J1D2
Locus tag E3165_RS27895 Protein ID WP_000450532.1
Coordinates 79356..79583 (-) Length 76 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E3165_RS27875 75197..75682 + 486 WP_000605866.1 hypothetical protein -
E3165_RS27880 75731..76462 + 732 WP_000782452.1 complement resistance protein TraT -
E3165_RS27885 76715..78949 + 2235 WP_171877397.1 type IV conjugative transfer system coupling protein TraD -
E3165_RS27890 78958..79356 - 399 WP_000911317.1 tRNA(fMet)-specific endonuclease VapC Toxin
E3165_RS27895 79356..79583 - 228 WP_000450532.1 toxin-antitoxin system antitoxin VapB Antitoxin

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - stcE / exeE / exeG / hlyC / hlyA / hlyB / hlyD / papC 1..119161 119161


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(5-125)

Antitoxin

(7-51)


Sequences


Toxin        


Download         Length: 133 a.a.        Molecular weight: 14857.14 Da        Isoelectric Point: 8.5264

>T121618 WP_000911317.1 NZ_CP038338:c79356-78958 [Escherichia coli O157:H7]
MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIIVTNNTREFERVGGLRIEDWS

Download         Length: 399 bp

>T121618 NZ_CP038338:c79356-78958 [Escherichia coli O157:H7]
ATGCTGAAGTTTATGCTCGATACCAACATCTGCATTTTTACGATAAAGAACAAACCCGCCAGTGTCAGGGAACGTTTTAA
CCTGAACCAGGGGAGAATGTGCATCAGTTCGGTCACTCTGATGGAGCTGATATATGGTGCAGAAAAAAGCCAGATGCCTG
AACGTAATCTCGCTGTGATCGAGGGATTTGTTTCCCGCATTGACGTTCTGGATTACGACGCTGCTGCTGCCACACACACC
GGCCAGATAAGAGCAGAACTTGCCCGTCAGGGACGCCCTGTCGGGCCATTTGATCAAATGATCGCAGGTCATGCCCGCAG
TCGGGGACTGATTATTGTGACTAATAACACCCGGGAATTTGAACGTGTGGGCGGCCTGAGAATTGAAGACTGGAGTTGA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8512.55 Da        Isoelectric Point: 4.5358

>AT121618 WP_000450532.1 NZ_CP038338:c79583-79356 [Escherichia coli O157:H7]
METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAGETWDEWFDGNSVSADFMDNREQPGMQERESF

Download         Length: 228 bp

>AT121618 NZ_CP038338:c79583-79356 [Escherichia coli O157:H7]
ATGGAAACCACCGTATTTCTCAGCAACCGCAGCCAGGCGGTCAGACTGCCAAAAGCGGTTGCATTGCCGGAAAACGTAAA
ACGCGTTGAAGTGATTGCTGTCGGACGAACCCGAATCATTACGCCTGCCGGAGAGACATGGGACGAATGGTTCGACGGAA
ACAGTGTCAGCGCTGATTTTATGGATAACAGGGAACAGCCTGGTATGCAGGAAAGGGAGTCATTCTGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure


Antitoxin

Source ID Structure
AlphaFold DB A0A829CHF5

References