Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 30397..31041 Replicon plasmid pLSU61-2
Accession NZ_CP038337
Organism Escherichia coli O157:H7 strain LSU61

Toxin (Protein)


Gene name hicA Uniprot ID A0A7L6LII1
Locus tag E3165_RS27380 Protein ID WP_000833471.1
Coordinates 30397..30579 (+) Length 61 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A7L6LHN8
Locus tag E3165_RS27385 Protein ID WP_000466317.1
Coordinates 30604..31041 (+) Length 146 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E3165_RS27340 25844..26167 - 324 WP_171877388.1 hypothetical protein -
E3165_RS27345 27402..27725 + 324 WP_000432877.1 hypothetical protein -
E3165_RS27350 27768..27992 + 225 WP_000866038.1 hypothetical protein -
E3165_RS27355 28088..28312 + 225 WP_000749828.1 hypothetical protein -
E3165_RS27360 28363..28629 + 267 WP_000868277.1 hypothetical protein -
E3165_RS27365 28619..28861 + 243 WP_001704452.1 transcriptional regulator -
E3165_RS27370 29300..29629 + 330 WP_000855123.1 hypothetical protein -
E3165_RS27375 29687..29965 + 279 WP_080171973.1 cobalamin biosynthesis protein CbiX -
E3165_RS27380 30397..30579 + 183 WP_000833471.1 type II toxin-antitoxin system HicA family toxin Toxin
E3165_RS27385 30604..31041 + 438 WP_000466317.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
E3165_RS27390 31070..31774 - 705 WP_001279862.1 hypothetical protein -
E3165_RS27395 31771..32532 - 762 WP_001097938.1 conjugal transfer protein TraL -
E3165_RS27400 32542..32949 - 408 WP_001190621.1 hypothetical protein -
E3165_RS27405 33295..33630 + 336 WP_000963832.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..37777 37777


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(4-60)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(7-138)


Sequences

Toxin        


Download         Length: 61 a.a.        Molecular weight: 6675.97 Da        Isoelectric Point: 11.0013

>T121612 WP_000833471.1 NZ_CP038337:30397-30579 [Escherichia coli O157:H7]
MKSADLLKELIAAGCELKRHKASSHQIWWSPITGKTFPVPHPKKDLPLGTVRSIRKMAGI

Download         Length: 183 bp

>T121612 NZ_CP038337:30397-30579 [Escherichia coli O157:H7]
ATGAAATCTGCTGACCTGTTGAAAGAACTGATAGCTGCTGGTTGTGAGTTGAAGCGGCATAAGGCGAGCAGCCACCAGAT
ATGGTGGTCGCCCATAACAGGAAAAACTTTTCCTGTGCCCCATCCGAAAAAAGACTTGCCACTGGGTACTGTCAGATCGA
TCAGAAAAATGGCGGGGATTTAA

Antitoxin


Download         Length: 146 a.a.        Molecular weight: 16227.10 Da        Isoelectric Point: 4.3135

>AT121612 WP_000466317.1 NZ_CP038337:30604-31041 [Escherichia coli O157:H7]
MFFSVGVETPKDDHTAYGITVPAFDRFDFGCVSAADTQSEIPVMAREAILAIVEEMVLSGSYSVDDIHDDGCLTYAANQD
YSHCDSWFVIDVDLSEIEGKQQRINIALPDVLIRRIDGFVRESGGVYRDRSHFLAQAARHELSYK

Download         Length: 438 bp

>AT121612 NZ_CP038337:30604-31041 [Escherichia coli O157:H7]
ATGTTTTTTTCTGTGGGTGTCGAAACACCGAAAGATGACCATACGGCATATGGTATAACCGTTCCGGCTTTTGATCGTTT
TGATTTTGGCTGTGTCTCTGCTGCCGATACTCAATCAGAAATTCCTGTGATGGCACGAGAAGCGATATTGGCTATCGTGG
AAGAAATGGTTTTAAGTGGTTCTTATTCTGTTGATGATATTCATGATGACGGCTGTTTAACATATGCCGCAAATCAGGAC
TATAGTCACTGCGATAGCTGGTTTGTAATTGATGTTGATTTGTCTGAAATTGAAGGTAAACAACAACGTATTAATATTGC
GTTGCCTGATGTGTTGATTCGTCGTATTGATGGATTTGTCAGAGAAAGTGGCGGTGTTTACAGAGATCGTAGCCATTTTC
TTGCTCAGGCCGCGCGGCATGAACTTTCATATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7L6LII1

Antitoxin

Source ID Structure
AlphaFold DB A0A7L6LHN8

References