Detailed information of TA system
Bioinformatically predictedOverview
TA module
Type | II | Classification (family/domain) | hicAB/HicA-HicB |
Location | 30397..31041 | Replicon | plasmid pLSU61-2 |
Accession | NZ_CP038337 | ||
Organism | Escherichia coli O157:H7 strain LSU61 |
Toxin (Protein)
Gene name | hicA | Uniprot ID | A0A7L6LII1 |
Locus tag | E3165_RS27380 | Protein ID | WP_000833471.1 |
Coordinates | 30397..30579 (+) | Length | 61 a.a. |
Antitoxin (Protein)
Gene name | hicB | Uniprot ID | A0A7L6LHN8 |
Locus tag | E3165_RS27385 | Protein ID | WP_000466317.1 |
Coordinates | 30604..31041 (+) | Length | 146 a.a. |
Genomic Context
Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
---|---|---|---|---|---|---|
E3165_RS27340 | 25844..26167 | - | 324 | WP_171877388.1 | hypothetical protein | - |
E3165_RS27345 | 27402..27725 | + | 324 | WP_000432877.1 | hypothetical protein | - |
E3165_RS27350 | 27768..27992 | + | 225 | WP_000866038.1 | hypothetical protein | - |
E3165_RS27355 | 28088..28312 | + | 225 | WP_000749828.1 | hypothetical protein | - |
E3165_RS27360 | 28363..28629 | + | 267 | WP_000868277.1 | hypothetical protein | - |
E3165_RS27365 | 28619..28861 | + | 243 | WP_001704452.1 | transcriptional regulator | - |
E3165_RS27370 | 29300..29629 | + | 330 | WP_000855123.1 | hypothetical protein | - |
E3165_RS27375 | 29687..29965 | + | 279 | WP_080171973.1 | cobalamin biosynthesis protein CbiX | - |
E3165_RS27380 | 30397..30579 | + | 183 | WP_000833471.1 | type II toxin-antitoxin system HicA family toxin | Toxin |
E3165_RS27385 | 30604..31041 | + | 438 | WP_000466317.1 | type II toxin-antitoxin system HicB family antitoxin | Antitoxin |
E3165_RS27390 | 31070..31774 | - | 705 | WP_001279862.1 | hypothetical protein | - |
E3165_RS27395 | 31771..32532 | - | 762 | WP_001097938.1 | conjugal transfer protein TraL | - |
E3165_RS27400 | 32542..32949 | - | 408 | WP_001190621.1 | hypothetical protein | - |
E3165_RS27405 | 33295..33630 | + | 336 | WP_000963832.1 | hypothetical protein | - |
Associated MGEs
MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
---|---|---|---|---|---|---|---|
- | inside | Non-Mobilizable plasmid | - | - | 1..37777 | 37777 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 61 a.a. Molecular weight: 6675.97 Da Isoelectric Point: 11.0013
>T121612 WP_000833471.1 NZ_CP038337:30397-30579 [Escherichia coli O157:H7]
MKSADLLKELIAAGCELKRHKASSHQIWWSPITGKTFPVPHPKKDLPLGTVRSIRKMAGI
MKSADLLKELIAAGCELKRHKASSHQIWWSPITGKTFPVPHPKKDLPLGTVRSIRKMAGI
Download Length: 183 bp
>T121612 NZ_CP038337:30397-30579 [Escherichia coli O157:H7]
ATGAAATCTGCTGACCTGTTGAAAGAACTGATAGCTGCTGGTTGTGAGTTGAAGCGGCATAAGGCGAGCAGCCACCAGAT
ATGGTGGTCGCCCATAACAGGAAAAACTTTTCCTGTGCCCCATCCGAAAAAAGACTTGCCACTGGGTACTGTCAGATCGA
TCAGAAAAATGGCGGGGATTTAA
ATGAAATCTGCTGACCTGTTGAAAGAACTGATAGCTGCTGGTTGTGAGTTGAAGCGGCATAAGGCGAGCAGCCACCAGAT
ATGGTGGTCGCCCATAACAGGAAAAACTTTTCCTGTGCCCCATCCGAAAAAAGACTTGCCACTGGGTACTGTCAGATCGA
TCAGAAAAATGGCGGGGATTTAA
Antitoxin
Download Length: 146 a.a. Molecular weight: 16227.10 Da Isoelectric Point: 4.3135
>AT121612 WP_000466317.1 NZ_CP038337:30604-31041 [Escherichia coli O157:H7]
MFFSVGVETPKDDHTAYGITVPAFDRFDFGCVSAADTQSEIPVMAREAILAIVEEMVLSGSYSVDDIHDDGCLTYAANQD
YSHCDSWFVIDVDLSEIEGKQQRINIALPDVLIRRIDGFVRESGGVYRDRSHFLAQAARHELSYK
MFFSVGVETPKDDHTAYGITVPAFDRFDFGCVSAADTQSEIPVMAREAILAIVEEMVLSGSYSVDDIHDDGCLTYAANQD
YSHCDSWFVIDVDLSEIEGKQQRINIALPDVLIRRIDGFVRESGGVYRDRSHFLAQAARHELSYK
Download Length: 438 bp
>AT121612 NZ_CP038337:30604-31041 [Escherichia coli O157:H7]
ATGTTTTTTTCTGTGGGTGTCGAAACACCGAAAGATGACCATACGGCATATGGTATAACCGTTCCGGCTTTTGATCGTTT
TGATTTTGGCTGTGTCTCTGCTGCCGATACTCAATCAGAAATTCCTGTGATGGCACGAGAAGCGATATTGGCTATCGTGG
AAGAAATGGTTTTAAGTGGTTCTTATTCTGTTGATGATATTCATGATGACGGCTGTTTAACATATGCCGCAAATCAGGAC
TATAGTCACTGCGATAGCTGGTTTGTAATTGATGTTGATTTGTCTGAAATTGAAGGTAAACAACAACGTATTAATATTGC
GTTGCCTGATGTGTTGATTCGTCGTATTGATGGATTTGTCAGAGAAAGTGGCGGTGTTTACAGAGATCGTAGCCATTTTC
TTGCTCAGGCCGCGCGGCATGAACTTTCATATAAATGA
ATGTTTTTTTCTGTGGGTGTCGAAACACCGAAAGATGACCATACGGCATATGGTATAACCGTTCCGGCTTTTGATCGTTT
TGATTTTGGCTGTGTCTCTGCTGCCGATACTCAATCAGAAATTCCTGTGATGGCACGAGAAGCGATATTGGCTATCGTGG
AAGAAATGGTTTTAAGTGGTTCTTATTCTGTTGATGATATTCATGATGACGGCTGTTTAACATATGCCGCAAATCAGGAC
TATAGTCACTGCGATAGCTGGTTTGTAATTGATGTTGATTTGTCTGAAATTGAAGGTAAACAACAACGTATTAATATTGC
GTTGCCTGATGTGTTGATTCGTCGTATTGATGGATTTGTCAGAGAAAGTGGCGGTGTTTACAGAGATCGTAGCCATTTTC
TTGCTCAGGCCGCGCGGCATGAACTTTCATATAAATGA
Similar Proteins
Only experimentally validated proteins are listed.
Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
---|
Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
---|
Structures
Toxin
Source | ID | Structure |
---|---|---|
AlphaFold DB | A0A7L6LII1 |
Antitoxin
Source | ID | Structure |
---|---|---|
AlphaFold DB | A0A7L6LHN8 |