Detailed information of TA system TA121611

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsxAB/MqsA(antitoxin)
• Location: 28363..28861
• Replicon: plasmid pLSU61-2
• Accession: NZ_CP038337
• Organism: Escherichia coli O157:H7 strain LSU61

Toxin (Protein)

• Gene name: TsxA
• Uniprot ID: A0A7L6LHZ8
• Locus tag: E3165_RS27360
• Protein ID: WP_000868277.1
• Coordinates: 28363..28629 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: TsxB
• Uniprot ID: -
• Locus tag: E3165_RS27365
• Protein ID: WP_001704452.1
• Coordinates: 28619..28861 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3165_RS27330	23829..24167	+	339	WP_000612441.1	helix-turn-helix domain-containing protein	-
E3165_RS27335	24695..25699	-	1005	WP_001208090.1	replication initiation protein	-
E3165_RS27340	25844..26167	-	324	WP_171877388.1	hypothetical protein	-
E3165_RS27345	27402..27725	+	324	WP_000432877.1	hypothetical protein	-
E3165_RS27350	27768..27992	+	225	WP_000866038.1	hypothetical protein	-
E3165_RS27355	28088..28312	+	225	WP_000749828.1	hypothetical protein	-
E3165_RS27360	28363..28629	+	267	WP_000868277.1	hypothetical protein	Toxin
E3165_RS27365	28619..28861	+	243	WP_001704452.1	transcriptional regulator	Antitoxin
E3165_RS27370	29300..29629	+	330	WP_000855123.1	hypothetical protein	-
E3165_RS27375	29687..29965	+	279	WP_080171973.1	cobalamin biosynthesis protein CbiX	-
E3165_RS27380	30397..30579	+	183	WP_000833471.1	type II toxin-antitoxin system HicA family toxin	-
E3165_RS27385	30604..31041	+	438	WP_000466317.1	type II toxin-antitoxin system HicB family antitoxin	-
E3165_RS27390	31070..31774	-	705	WP_001279862.1	hypothetical protein	-
E3165_RS27395	31771..32532	-	762	WP_001097938.1	conjugal transfer protein TraL	-
E3165_RS27400	32542..32949	-	408	WP_001190621.1	hypothetical protein	-
E3165_RS27405	33295..33630	+	336	WP_000963832.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..37777	37777

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (8-37)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10205.74 Da
• Isoelectric Point: 10.4299

>T121611 WP_000868277.1 NZ_CP038337:28363-28629 [Escherichia coli O157:H7]
MKVRKGDRQYYLNKEGNMFHLVKRVKTFSKSATLGKTKATVKTVADLVFNEKAFDTIDFSRDGLRENDKEIVSMMIQEMN
NERGKNVN

Nucleotide

• Length: 267 bp

>T121611 NZ_CP038337:28363-28629 [Escherichia coli O157:H7]
ATGAAGGTACGAAAAGGTGACAGGCAATATTATTTGAATAAAGAAGGTAATATGTTTCATCTTGTGAAACGGGTGAAGAC
CTTTTCTAAATCTGCGACTTTAGGAAAAACGAAAGCAACGGTGAAAACTGTTGCTGATCTTGTTTTTAATGAGAAGGCAT
TCGACACAATAGACTTTTCCAGAGATGGATTAAGAGAGAATGATAAAGAAATTGTTTCTATGATGATTCAGGAAATGAAC
AATGAACGGGGAAAAAATGTTAACTGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9250.78 Da
• Isoelectric Point: 9.2372

>AT121611 WP_001704452.1 NZ_CP038337:28619-28861 [Escherichia coli O157:H7]
MLTENTPENIKRLRKKIGLTQKECAEIFSMTPRTWRRKEEPVGTVSGTALTPVEFKYLQLLAGEHPEYVLCKRDKPKCSE

Nucleotide

• Length: 243 bp

>AT121611 NZ_CP038337:28619-28861 [Escherichia coli O157:H7]
ATGTTAACTGAAAACACACCAGAAAACATCAAACGTCTGCGGAAAAAAATTGGTCTGACGCAGAAAGAATGTGCCGAAAT
ATTCAGCATGACTCCGCGAACTTGGCGTCGCAAGGAAGAACCTGTCGGAACTGTAAGTGGTACAGCACTTACACCTGTGG
AGTTTAAATATCTTCAGCTTTTAGCTGGGGAACATCCAGAATACGTTCTGTGCAAAAGGGACAAGCCTAAATGTAGTGAA
TGA

Structures

Toxin

• AlphaFold DB: A0A7L6LHZ8