Detailed information of TA system TA121606

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 4679307..4679857
• Replicon: chromosome
• Accession: NZ_CP038336
• Organism: Escherichia coli O157:H7 strain LSU61

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: D3H432
• Locus tag: E3165_RS23315
• Protein ID: WP_001160975.1
• Coordinates: 4679307..4679621 (-)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: V0VEX8
• Locus tag: E3165_RS23320
• Protein ID: WP_000125566.1
• Coordinates: 4679624..4679857 (-)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3165_RS23290	4674982..4676268	+	1287	WP_000800454.1	peptidylprolyl isomerase SurA	-
E3165_RS23295	4676268..4677257	+	990	WP_000241259.1	4-hydroxythreonine-4-phosphate dehydrogenase PdxA	-
E3165_RS23300	4677254..4678075	+	822	WP_001065380.1	16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA	-
E3165_RS23305	4678078..4678455	+	378	WP_000610901.1	Co2+/Mg2+ efflux protein ApaG	-
E3165_RS23310	4678462..4679310	+	849	WP_000257187.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH	-
E3165_RS23315	4679307..4679621	-	315	WP_001160975.1	CcdB family protein	Toxin
E3165_RS23320	4679624..4679857	-	234	WP_000125566.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
E3165_RS23325	4679943..4680422	-	480	WP_000624375.1	type 3 dihydrofolate reductase	-
E3165_RS23330	4680614..4682476	-	1863	WP_000377184.1	glutathione-regulated potassium-efflux system protein KefC	-
E3165_RS23335	4682469..4682999	-	531	WP_000600700.1	glutathione-regulated potassium-efflux system oxidoreductase KefF	-
E3165_RS23340	4683107..4684438	-	1332	WP_001183177.1	MFS transporter	-
E3165_RS23345	4684496..4684783	-	288	WP_000203741.1	ferredoxin-like protein FixX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-103)

Antitoxin

• Post-segregation antitoxin CcdA (8-77)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11711.76 Da
• Isoelectric Point: 9.8517

>T121606 WP_001160975.1 NZ_CP038336:c4679621-4679307 [Escherichia coli O157:H7]
MQFTVYRSRSRNAAFPFVIDVTSDIIGVINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Nucleotide

• Length: 315 bp

>T121606 NZ_CP038336:c4679621-4679307 [Escherichia coli O157:H7]
ATGCAATTTACGGTATACCGCAGTCGCAGCAGGAACGCCGCGTTTCCCTTTGTTATCGATGTTACCAGCGACATTATTGG
TGTGATTAATCGCCGTATTGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8530.53 Da
• Isoelectric Point: 5.8062

>AT121606 WP_000125566.1 NZ_CP038336:c4679857-4679624 [Escherichia coli O157:H7]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Nucleotide

• Length: 234 bp

>AT121606 NZ_CP038336:c4679857-4679624 [Escherichia coli O157:H7]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CTGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Structures

Antitoxin

• AlphaFold DB: A0A7U9LTM9