Detailed information of TA system TA121597

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2824029..2824667
• Replicon: chromosome
• Accession: NZ_CP038336
• Organism: Escherichia coli O157:H7 strain LSU61

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: E3165_RS14105
• Protein ID: WP_000813794.1
• Coordinates: 2824491..2824667 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: E3165_RS14100
• Protein ID: WP_001270286.1
• Coordinates: 2824029..2824445 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3165_RS14080	2819931..2820497	-	567	Protein_2756	extracellular solute-binding protein	-
E3165_RS14085	2820560..2821711	+	1152	WP_001254932.1	IS30-like element IS30 family transposase	-
E3165_RS14090	2821718..2822299	-	582	Protein_2758	extracellular solute-binding protein	-
E3165_RS14095	2822544..2823950	-	1407	WP_000760654.1	PLP-dependent aminotransferase family protein	-
E3165_RS14100	2824029..2824445	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
E3165_RS14105	2824491..2824667	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
E3165_RS14110	2824889..2825119	+	231	WP_000494244.1	YncJ family protein	-
E3165_RS14115	2825211..2827172	-	1962	WP_001303492.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
E3165_RS14120	2827245..2827781	-	537	WP_000429145.1	DNA-binding transcriptional regulator SutR	-
E3165_RS14125	2827834..2829045	+	1212	WP_071987739.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2820560..2821711	1151

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T121597 WP_000813794.1 NZ_CP038336:c2824667-2824491 [Escherichia coli O157:H7]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T121597 NZ_CP038336:c2824667-2824491 [Escherichia coli O157:H7]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT121597 WP_001270286.1 NZ_CP038336:c2824445-2824029 [Escherichia coli O157:H7]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT121597 NZ_CP038336:c2824445-2824029 [Escherichia coli O157:H7]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACAGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAAGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• PDB: 6HPB