121593

Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) paaR-paaA-parE/-
Location 2545665..2546136 Replicon chromosome
Accession NZ_CP038336
Organism Escherichia coli O157:H7 strain LSU61

Toxin (Protein)


Gene name parE_1 Uniprot ID A0A0D7C2L1
Locus tag E3165_RS12540 Protein ID WP_001303511.1
Coordinates 2545665..2545943 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name paaA Uniprot ID Q8XAD5
Locus tag E3165_RS12545 Protein ID WP_001302048.1
Coordinates 2545945..2546136 (-) Length 64 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E3165_RS12510 2541259..2543685 - 2427 Protein_2449 3'-5' exoribonuclease -
E3165_RS12515 2543778..2543969 - 192 WP_001090200.1 DUF1482 family protein -
E3165_RS12520 2543966..2544154 - 189 WP_000449192.1 cell division inhibition protein DicB -
E3165_RS12525 2544555..2544719 + 165 WP_001331716.1 hypothetical protein -
E3165_RS12530 2544723..2544941 - 219 WP_001171930.1 hypothetical protein -
E3165_RS12535 2545013..2545312 - 300 WP_001240334.1 hypothetical protein -
E3165_RS12540 2545665..2545943 - 279 WP_001303511.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
E3165_RS12545 2545945..2546136 - 192 WP_001302048.1 hypothetical protein Antitoxin
E3165_RS12550 2546157..2546528 - 372 WP_001169686.1 hypothetical protein -
E3165_RS12555 2546626..2546928 + 303 WP_000172738.1 transcriptional regulator -
E3165_RS12560 2546925..2547350 + 426 WP_000693943.1 Rha family transcriptional regulator -
E3165_RS12565 2547373..2548335 + 963 WP_000095669.1 helix-turn-helix domain-containing protein -
E3165_RS12570 2548342..2549082 + 741 WP_000788938.1 ATP-binding protein -
E3165_RS12575 2549108..2549878 + 771 WP_000450876.1 DUF1627 domain-containing protein -
E3165_RS12580 2549894..2550289 + 396 WP_001118159.1 DUF977 family protein -
E3165_RS12585 2550346..2550870 + 525 WP_171877379.1 DUF551 domain-containing protein -
E3165_RS12590 2550877..2550930 + 54 Protein_2465 DUF551 domain-containing protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - nleA/espI / nleH2 / nleF / espM1 / paa / nleG7' / nleC 2530821..2698192 167371


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(4-91)

Antitoxin


No domain identified.



Sequences


Toxin        


Download         Length: 93 a.a.        Molecular weight: 10661.25 Da        Isoelectric Point: 5.5647

>T121593 WP_001303511.1 NZ_CP038336:c2545943-2545665 [Escherichia coli O157:H7]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Download         Length: 279 bp

>T121593 NZ_CP038336:c2545943-2545665 [Escherichia coli O157:H7]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin


Download         Length: 64 a.a.        Molecular weight: 7463.47 Da        Isoelectric Point: 8.6828

>AT121593 WP_001302048.1 NZ_CP038336:c2546136-2545945 [Escherichia coli O157:H7]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Download         Length: 192 bp

>AT121593 NZ_CP038336:c2546136-2545945 [Escherichia coli O157:H7]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
PDB 5CZF
PDB 5CW7
PDB 5CZE


Antitoxin

Source ID Structure
AlphaFold DB A0A829CTP0

References