Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | relBE/RHH(antitoxin) |
| Location | 73463..74022 | Replicon | plasmid pN8B7-1 |
| Accession | NZ_CP038335 | ||
| Organism | Escherichia coli O157:H7 strain N8B7-2 | ||
Toxin (Protein)
| Gene name | relE | Uniprot ID | O82928 |
| Locus tag | E4U68_RS28190 | Protein ID | WP_000421248.1 |
| Coordinates | 73747..74022 (+) | Length | 92 a.a. |
Antitoxin (Protein)
| Gene name | relB | Uniprot ID | C1J8K5 |
| Locus tag | E4U68_RS28185 | Protein ID | WP_001178089.1 |
| Coordinates | 73463..73747 (+) | Length | 95 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| E4U68_RS28145 | 68655..69215 | + | 561 | WP_000840472.1 | fertility inhibition protein FinO | - |
| E4U68_RS28150 | 69348..69560 | + | 213 | WP_001302189.1 | ANR family transcriptional regulator | - |
| E4U68_RS28155 | 69805..70266 | + | 462 | WP_001233853.1 | thermonuclease family protein | - |
| E4U68_RS28160 | 70312..70521 | + | 210 | WP_001302200.1 | hemolysin expression modulator Hha | - |
| E4U68_RS28165 | 70559..70897 | + | 339 | WP_000766796.1 | hypothetical protein | - |
| E4U68_RS28170 | 71137..71391 | + | 255 | WP_000083831.1 | replication regulatory protein RepA | - |
| E4U68_RS28175 | 71627..71701 | + | 75 | WP_001370046.1 | RepA leader peptide Tap | - |
| E4U68_RS28180 | 71694..72551 | + | 858 | WP_000130945.1 | incFII family plasmid replication initiator RepA | - |
| E4U68_RS28185 | 73463..73747 | + | 285 | WP_001178089.1 | ribbon-helix-helix domain-containing protein | Antitoxin |
| E4U68_RS28190 | 73747..74022 | + | 276 | WP_000421248.1 | type II toxin-antitoxin system RelE/ParE family toxin | Toxin |
| E4U68_RS28195 | 74117..74323 | + | 207 | WP_001105064.1 | hypothetical protein | - |
| E4U68_RS28200 | 74423..74638 | - | 216 | Protein_81 | transposase | - |
| E4U68_RS28205 | 74682..75248 | - | 567 | Protein_82 | IS3-like element IS600 family transposase | - |
| E4U68_RS28210 | 75248..75664 | - | 417 | Protein_83 | transposase zinc-binding domain-containing protein | - |
| E4U68_RS28215 | 75664..75849 | - | 186 | WP_001302179.1 | hypothetical protein | - |
| E4U68_RS28220 | 76026..78236 | + | 2211 | WP_000592771.1 | catalase/peroxidase KatP | - |
| E4U68_RS28225 | 78280..78669 | + | 390 | WP_001172748.1 | cytochrome b562 family protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | - | stcE / exeE / exeG / hlyC / hlyA / hlyB / hlyD / toxB / espP | 1..94694 | 94694 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 92 a.a. Molecular weight: 10735.42 Da Isoelectric Point: 9.9324
>T121575 WP_000421248.1 NZ_CP038335:73747-74022 [Escherichia coli O157:H7]
MELKWSSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIY
VLRLWHTRENR
MELKWSSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIY
VLRLWHTRENR
Download Length: 276 bp
>T121575 NZ_CP038335:73747-74022 [Escherichia coli O157:H7]
ATGGAACTGAAGTGGAGCAGTAAGGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTAGCCAGTAAACCTGC
TGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGGGAACAGTTATTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTAATGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTAG
ATGGAACTGAAGTGGAGCAGTAAGGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTAGCCAGTAAACCTGC
TGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGGGAACAGTTATTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTAATGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTAG
Antitoxin
Download Length: 95 a.a. Molecular weight: 10474.89 Da Isoelectric Point: 5.6558
>AT121575 WP_001178089.1 NZ_CP038335:73463-73747 [Escherichia coli O157:H7]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR
Download Length: 285 bp
>AT121575 NZ_CP038335:73463-73747 [Escherichia coli O157:H7]
ATGCAAATGAAAAACAATACCGCACAAGCAACAAAAGTCATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGTTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATTGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA
ATGCAAATGAAAAACAATACCGCACAAGCAACAAAAGTCATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGTTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATTGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A2Z6FAZ0 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A731IHA8 |