Detailed information of TA system TA121573

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsxAB/Xre(antitoxin)
• Location: 7372..7873
• Replicon: plasmid pN8B7-2
• Accession: NZ_CP038334
• Organism: Escherichia coli O157:H7 strain N8B7-2

Toxin (Protein)

• Gene name: TsxA
• Uniprot ID: A0A6X6XTU7
• Locus tag: E4U68_RS27450
• Protein ID: WP_000702245.1
• Coordinates: 7372..7632 (+)
• Length: 87 a.a.

Antitoxin (Protein)

• Gene name: TsxB
• Uniprot ID: -
• Locus tag: E4U68_RS27455
• Protein ID: WP_001132407.1
• Coordinates: 7622..7873 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E4U68_RS27405	2614..2775	+	162	WP_001128311.1	hypothetical protein	-
E4U68_RS27410	2969..3130	+	162	WP_001366886.1	type I toxin-antitoxin system Hok family toxin	-
E4U68_RS27415	3280..3876	+	597	WP_000125165.1	hypothetical protein	-
E4U68_RS27420	3894..4064	-	171	WP_000186151.1	hypothetical protein	-
E4U68_RS27425	4252..4917	-	666	WP_000532148.1	P-loop NTPase	-
E4U68_RS27430	5515..5844	+	330	WP_000855121.1	hypothetical protein	-
E4U68_RS27435	6425..6748	+	324	WP_000427402.1	hypothetical protein	-
E4U68_RS27440	6789..6998	+	210	WP_000866037.1	hypothetical protein	-
E4U68_RS27445	7096..7320	+	225	WP_000814383.1	hypothetical protein	-
E4U68_RS27450	7372..7632	+	261	WP_000702245.1	hypothetical protein	Toxin
E4U68_RS27455	7622..7873	+	252	WP_001132407.1	transcriptional regulator	Antitoxin
E4U68_RS27460	8423..8701	+	279	WP_000545937.1	hypothetical protein	-
E4U68_RS27465	9088..9270	-	183	WP_001401700.1	hypothetical protein	-
E4U68_RS27470	9828..10010	+	183	WP_000833470.1	type II toxin-antitoxin system HicA family toxin	-
E4U68_RS27475	10035..10472	+	438	WP_000466318.1	type II toxin-antitoxin system HicB family antitoxin	-
E4U68_RS27480	10602..10922	-	321	WP_171880298.1	hypothetical protein	-
E4U68_RS27485	10933..11196	-	264	WP_000224227.1	hypothetical protein	-
E4U68_RS27490	11198..11689	-	492	WP_001167293.1	hypothetical protein	-
E4U68_RS27495	11692..12144	-	453	WP_000206703.1	hypothetical protein	-
E4U68_RS27500	12164..12871	-	708	WP_000108795.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..58668	58668

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (8-37)

Sequences

Toxin

Protein

• Length: 87 a.a.
• Molecular weight: 9818.23 Da
• Isoelectric Point: 9.5163

>T121573 WP_000702245.1 NZ_CP038334:7372-7632 [Escherichia coli O157:H7]
MKIRKGDRQYYLNKEGDTFHLVKRVKTFSKSATLGKTKATVKTVADLVFHEEAFDTIDFASDGLRENDKEIVSMMIQEMS
EGKNAK

Nucleotide

• Length: 261 bp

>T121573 NZ_CP038334:7372-7632 [Escherichia coli O157:H7]
ATGAAGATAAGAAAAGGTGACAGACAATATTATCTGAATAAAGAAGGTGATACGTTTCATCTGGTGAAGCGAGTAAAAAC
TTTTTCTAAGTCTGCGACTTTAGGAAAAACGAAAGCAACGGTGAAAACTGTTGCTGATCTTGTTTTTCATGAGGAGGCAT
TCGACACTATCGACTTTGCCAGCGACGGATTAAGAGAGAATGATAAAGAAATTGTTTCTATGATGATTCAGGAAATGAGT
GAGGGAAAAAATGCCAAATGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9404.77 Da
• Isoelectric Point: 9.8120

>AT121573 WP_001132407.1 NZ_CP038334:7622-7873 [Escherichia coli O157:H7]
MPNENTPENIKRLRQKIGLTQKECAEIFSMSPRTWRRKEEPVGTASGTALTPVEFKFLLLLAGEHPDYVLCKRNKKNSDS
GSN

Nucleotide

• Length: 252 bp

>AT121573 NZ_CP038334:7622-7873 [Escherichia coli O157:H7]
ATGCCAAATGAAAACACACCAGAAAACATTAAACGTCTGCGGCAAAAAATTGGCCTGACACAGAAAGAATGTGCTGAAAT
ATTCAGCATGAGTCCGCGTACCTGGCGTCGCAAAGAAGAACCTGTCGGAACTGCAAGCGGTACAGCACTTACACCAGTAG
AGTTTAAATTTCTTCTGCTTTTAGCTGGAGAACACCCTGATTATGTACTTTGTAAAAGGAATAAAAAGAATTCTGATTCT
GGAAGTAATTAA

Structures

Toxin

• AlphaFold DB: A0A6X6XTU7