Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/RelE-HTH_XRE
Location 3728350..3729055 Replicon chromosome
Accession NZ_CP038333
Organism Escherichia coli O157:H7 strain N8B7-2

Toxin (Protein)


Gene name relE Uniprot ID Q8X3N0
Locus tag E4U68_RS19150 Protein ID WP_000539519.1
Coordinates 3728350..3728736 (+) Length 129 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID -
Locus tag E4U68_RS19155 Protein ID WP_001280945.1
Coordinates 3728726..3729055 (+) Length 110 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E4U68_RS19130 3724354..3724980 + 627 WP_001295292.1 glutathione S-transferase GstB -
E4U68_RS19135 3724977..3726092 - 1116 WP_000554959.1 aldose sugar dehydrogenase YliI -
E4U68_RS19140 3726203..3726586 - 384 WP_000497137.1 biofilm formation regulator BssR -
E4U68_RS19145 3726799..3728124 + 1326 WP_000049367.1 30S ribosomal protein S12 methylthiotransferase RimO -
E4U68_RS19150 3728350..3728736 + 387 WP_000539519.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
E4U68_RS19155 3728726..3729055 + 330 WP_001280945.1 DNA-binding transcriptional regulator Antitoxin
E4U68_RS19160 3729125..3730453 - 1329 WP_000086905.1 GGDEF domain-containing protein -
E4U68_RS19165 3730461..3732809 - 2349 WP_000950320.1 EAL domain-containing protein -
E4U68_RS19170 3732987..3733898 - 912 WP_001236044.1 glutathione ABC transporter permease GsiD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-2

(1-124)

Antitoxin

Cro/C1-type helix-turn-helix domain

(53-94)


Sequences

Toxin        


Download         Length: 129 a.a.        Molecular weight: 14279.42 Da        Isoelectric Point: 9.9296

>T121564 WP_000539519.1 NZ_CP038333:3728350-3728736 [Escherichia coli O157:H7]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGSKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Download         Length: 387 bp

>T121564 NZ_CP038333:3728350-3728736 [Escherichia coli O157:H7]
ATGGGTGTGTATAAGGCAAGGCGATTCAGCCAAAGCACGAAAAAACTTGGTATTCACGATAAAGTTCTCATGGCTGCCGC
TGAAGAAGTGATGCAAGGCATATGGGAGGCTGATTTAGGTAGCGGTGTCATCAAAAAACGGTTACCCCTCCAACAAGGAA
AGAGTGGTGGAGCCAGAACAATTATCTTTTTTAAATCAGCAAATCATGTGTTTTTTTATGATGGTTGGTCCAAGAGTGGT
CTAAGCAGTAAAGGAAGCAAAGAAATCGAAGATGATGAACTGGCAGCCTACAAAAAAATGGCAAATGCATTTCTGGCCTT
TAGCAATAAGCAAATAGAGGATTTGATCGAAACTGGCTTCTTGATTGAGGTGAAAAATGAGCGATAA

Antitoxin


Download         Length: 110 a.a.        Molecular weight: 11982.08 Da        Isoelectric Point: 10.0101

>AT121564 WP_001280945.1 NZ_CP038333:3728726-3729055 [Escherichia coli O157:H7]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Download         Length: 330 bp

>AT121564 NZ_CP038333:3728726-3729055 [Escherichia coli O157:H7]
ATGAGCGATAAATTACAAATGATGCTGGAAGATGCAAAGGAACTGAATGCACTGGGCCTGATGTCTGATAGTGAGGTTGA
TGCCATTGCCGTCATGGTCAAAGCCCGTGAAGTCAGCGCCCGTATAGCCAAAGTCAAATCAATGACGGGTGAAGAGATCA
AAGCCATCCGCACCCGATACGGCATGTCACAATCCGTGCTGGCACATACAATGGGAATGTCAAAAGAAAGTGTCTCGAAA
TGGGAACGTAATGAAATAAAACCGAGCGGCCCGGCACTACGTATTTTGAATACATTGGCAATAAAAGGGCCGGAAGTTTT
TGCAAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References