Detailed information of TA system TA121548

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 2141857..2142652
• Replicon: chromosome
• Accession: NZ_CP038333
• Organism: Escherichia coli O157:H7 strain N8B7-2

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: B7UP43
• Locus tag: E4U68_RS10715
• Protein ID: WP_000854914.1
• Coordinates: 2142278..2142652 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: E4U68_RS10710
• Protein ID: WP_001416641.1
• Coordinates: 2141857..2142231 (+)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E4U68_RS10680	2137212..2138117	+	906	WP_000203541.1	chemotaxis protein	-
E4U68_RS10685	2138114..2139184	+	1071	WP_000102674.1	patatin-like phospholipase family protein	-
E4U68_RS10690	2139524..2140342	+	819	WP_001234682.1	DUF945 domain-containing protein	-
E4U68_RS10695	2140433..2140918	+	486	WP_000214398.1	antirestriction protein	-
E4U68_RS10700	2140934..2141410	+	477	WP_001186738.1	RadC family protein	-
E4U68_RS10705	2141473..2141694	+	222	WP_001220314.1	DUF987 domain-containing protein	-
E4U68_RS10710	2141857..2142231	+	375	WP_001416641.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
E4U68_RS10715	2142278..2142652	+	375	WP_000854914.1	TA system toxin CbtA family protein	Toxin
E4U68_RS10720	2142649..2143140	+	492	WP_000976853.1	hypothetical protein	-
E4U68_RS10725	2143152..2143349	+	198	WP_000839282.1	DUF957 domain-containing protein	-
E4U68_RS10730	2143434..2144276	+	843	WP_001280481.1	DUF4942 domain-containing protein	-
E4U68_RS10740	2144747..2145685	+	939	WP_000351284.1	glyoxylate/hydroxypyruvate reductase GhrA	-
E4U68_RS10745	2145740..2146477	+	738	WP_000283664.1	zinc-binding phosphatase	-
E4U68_RS10750	2146501..2147055	+	555	WP_001001917.1	molecular chaperone YcdY	-
E4U68_RS10755	2147157..2147648	+	492	WP_001301587.1	DUF1097 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 14117.17 Da
• Isoelectric Point: 7.7761

>T121548 WP_000854914.1 NZ_CP038333:2142278-2142652 [Escherichia coli O157:H7]
MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T121548 NZ_CP038333:2142278-2142652 [Escherichia coli O157:H7]
ATGAAAACATTATCCGACACACATGTACGGGAGGTATCTCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAT
CCGACTGCTGGACCAGCATTATGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTTAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACCTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 13778.61 Da
• Isoelectric Point: 6.6249

>AT121548 WP_001416641.1 NZ_CP038333:2141857-2142231 [Escherichia coli O157:H7]
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRYQHTVTLNAKGLTCEADTLGFCGYVYLAVYPTPAPATTS

Nucleotide

• Length: 375 bp

>AT121548 NZ_CP038333:2141857-2142231 [Escherichia coli O157:H7]
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGATGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGCGCTCGTCTGGTGCAAGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGATATCAGCATACCGTCACGCTGAATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCTTCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCAGCACCCGCAACCACCTCATAA

Structures

Toxin

• AlphaFold DB: A0A7U9LXR5