Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | relBE/RHH(antitoxin) |
| Location | 46551..47110 | Replicon | plasmid pNE1092-1 |
| Accession | NZ_CP038332 | ||
| Organism | Escherichia coli O157:H7 strain NE 1092-2 | ||
Toxin (Protein)
| Gene name | relE | Uniprot ID | O82928 |
| Locus tag | E4U73_RS28810 | Protein ID | WP_000421248.1 |
| Coordinates | 46551..46826 (-) | Length | 92 a.a. |
Antitoxin (Protein)
| Gene name | relB | Uniprot ID | C1J8K5 |
| Locus tag | E4U73_RS28815 | Protein ID | WP_001178089.1 |
| Coordinates | 46826..47110 (-) | Length | 95 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| E4U73_RS28775 | 41904..42293 | - | 390 | WP_001172748.1 | cytochrome b562 family protein | - |
| E4U73_RS28780 | 42337..44547 | - | 2211 | WP_000592771.1 | catalase/peroxidase KatP | - |
| E4U73_RS28785 | 44724..44909 | + | 186 | WP_001302179.1 | hypothetical protein | - |
| E4U73_RS28790 | 44909..45325 | + | 417 | Protein_37 | transposase zinc-binding domain-containing protein | - |
| E4U73_RS28795 | 45325..45891 | + | 567 | Protein_38 | IS3-like element IS600 family transposase | - |
| E4U73_RS28800 | 45935..46150 | + | 216 | Protein_39 | transposase | - |
| E4U73_RS28805 | 46250..46456 | - | 207 | WP_001105064.1 | hypothetical protein | - |
| E4U73_RS28810 | 46551..46826 | - | 276 | WP_000421248.1 | type II toxin-antitoxin system RelE/ParE family toxin | Toxin |
| E4U73_RS28815 | 46826..47110 | - | 285 | WP_001178089.1 | ribbon-helix-helix domain-containing protein | Antitoxin |
| E4U73_RS28820 | 48022..48879 | - | 858 | WP_000130945.1 | incFII family plasmid replication initiator RepA | - |
| E4U73_RS28825 | 48872..48946 | - | 75 | WP_001370046.1 | RepA leader peptide Tap | - |
| E4U73_RS28830 | 49182..49436 | - | 255 | WP_000083831.1 | replication regulatory protein RepA | - |
| E4U73_RS28835 | 49676..50014 | - | 339 | WP_000766796.1 | hypothetical protein | - |
| E4U73_RS28840 | 50052..50261 | - | 210 | WP_001302200.1 | hemolysin expression modulator Hha | - |
| E4U73_RS28845 | 50307..50768 | - | 462 | WP_001233853.1 | thermonuclease family protein | - |
| E4U73_RS28850 | 51013..51225 | - | 213 | WP_001302189.1 | ANR family transcriptional regulator | - |
| E4U73_RS28855 | 51358..51918 | - | 561 | WP_000840472.1 | fertility inhibition protein FinO | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | - | hlyD / hlyB / hlyA / hlyC / exeG / exeE / stcE / espP / toxB | 1..92495 | 92495 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 92 a.a. Molecular weight: 10735.42 Da Isoelectric Point: 9.9324
>T121539 WP_000421248.1 NZ_CP038332:c46826-46551 [Escherichia coli O157:H7]
MELKWSSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIY
VLRLWHTRENR
MELKWSSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIY
VLRLWHTRENR
Download Length: 276 bp
>T121539 NZ_CP038332:c46826-46551 [Escherichia coli O157:H7]
ATGGAACTGAAGTGGAGCAGTAAAGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTAGCCAGTAAACCTGC
TGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGGGAACAGTTATTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTAATGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTAG
ATGGAACTGAAGTGGAGCAGTAAAGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTAGCCAGTAAACCTGC
TGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGGGAACAGTTATTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTAATGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTAG
Antitoxin
Download Length: 95 a.a. Molecular weight: 10474.89 Da Isoelectric Point: 5.6558
>AT121539 WP_001178089.1 NZ_CP038332:c47110-46826 [Escherichia coli O157:H7]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR
Download Length: 285 bp
>AT121539 NZ_CP038332:c47110-46826 [Escherichia coli O157:H7]
ATGCAAATGAAAAACAATACCGCACAAGCAACAAAAGTCATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGTTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATTGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA
ATGCAAATGAAAAACAATACCGCACAAGCAACAAAAGTCATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGTTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATTGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A2Z6FAZ0 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A731IHA8 |