Detailed information of TA system TA121537

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PanAT/DUF4065(antitoxin)
• Location: 5249484..5250472
• Replicon: chromosome
• Accession: NZ_CP038328
• Organism: Escherichia coli O157:H7 strain NE 1092-2

Toxin (Protein)

• Gene name: panT
• Uniprot ID: -
• Locus tag: E4U73_RS26325
• Protein ID: WP_014640052.1
• Coordinates: 5249484..5249870 (-)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: panA
• Uniprot ID: A0A0J5Z6D5
• Locus tag: E4U73_RS26330
• Protein ID: WP_000458584.1
• Coordinates: 5249900..5250472 (-)
• Length: 191 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E4U73_RS26275	5244490..5245314	+	825	WP_000081287.1	DUF2303 family protein	-
E4U73_RS26280	5245442..5245978	+	537	WP_096850447.1	5'-deoxynucleotidase	-
E4U73_RS26285	5245969..5246319	+	351	WP_001242740.1	hypothetical protein	-
E4U73_RS26290	5246316..5246789	+	474	WP_000145671.1	class I SAM-dependent methyltransferase	-
E4U73_RS26295	5246936..5247403	+	468	WP_000829413.1	hypothetical protein	-
E4U73_RS26300	5247405..5247596	+	192	WP_001014294.1	hypothetical protein	-
E4U73_RS26305	5247599..5248333	+	735	WP_001094871.1	DUF551 domain-containing protein	-
E4U73_RS26310	5248333..5248905	+	573	WP_001061339.1	3'-5' exoribonuclease	-
E4U73_RS26315	5248942..5249223	+	282	WP_001093919.1	pyocin activator PrtN family protein	-
E4U73_RS26320	5249271..5249444	-	174	WP_000390073.1	hypothetical protein	-
E4U73_RS26325	5249484..5249870	-	387	WP_014640052.1	hypothetical protein	Toxin
E4U73_RS26330	5249900..5250472	-	573	WP_000458584.1	SocA family protein	Antitoxin
E4U73_RS26335	5250708..5250830	-	123	WP_001300034.1	hypothetical protein	-
E4U73_RS26340	5251192..5252148	-	957	WP_000912588.1	DUF2713 family protein	-
E4U73_RS26345	5252467..5252982	+	516	WP_001301681.1	zinc uptake transcriptional repressor Zur	-
E4U73_RS26350	5253024..5253233	-	210	WP_001030593.1	CsbD family protein	-
E4U73_RS26355	5253349..5254674	-	1326	WP_001301558.1	MATE family efflux transporter DinF	-
E4U73_RS26360	5254747..5255355	-	609	WP_000646078.1	transcriptional repressor LexA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	nleG7'	5196597..5255355	58758

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Antitoxin SocA-like, Panacea domain (27-137)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14465.40 Da
• Isoelectric Point: 5.4833

>T121537 WP_014640052.1 NZ_CP038328:c5249870-5249484 [Escherichia coli O157:H7]
VLAPTGPCNHLHVICNDPVYYPVNDCYCVLVVNISSIKDGVPHDPSCVLNSGDHRFIKHPSYVVYAEAIIWRVDNMVRKQ
RSGEISVHDDMPEATFNRILDGFDISDEVTPKNLKFKNKYCVSSIDDE

Nucleotide

• Length: 387 bp

>T121537 NZ_CP038328:c5249870-5249484 [Escherichia coli O157:H7]
GTGCTTGCCCCAACTGGACCATGCAATCATCTTCATGTGATTTGTAATGATCCTGTTTATTACCCCGTTAACGATTGTTA
TTGTGTTTTAGTTGTTAATATTTCTAGTATCAAGGATGGTGTCCCCCACGATCCGTCTTGCGTCTTGAATTCTGGTGATC
ATCGCTTTATCAAGCATCCAAGTTATGTTGTTTACGCTGAAGCTATAATTTGGCGAGTGGATAACATGGTTAGAAAGCAG
CGATCGGGTGAGATTTCTGTTCATGATGATATGCCAGAAGCTACATTCAATAGAATTCTGGACGGTTTTGATATCTCTGA
TGAAGTTACGCCAAAGAACCTTAAATTTAAAAATAAATATTGCGTATCATCTATTGATGATGAGTAA

Antitoxin

Protein

• Length: 191 a.a.
• Molecular weight: 21973.21 Da
• Isoelectric Point: 6.0283

>AT121537 WP_000458584.1 NZ_CP038328:c5250472-5249900 [Escherichia coli O157:H7]
MFCEEKVAQMAAYLLLKRGGRMAYLKLMKLLYLSNRQSILKHGRMIGEDSLYSMKFGPVMSNTLNLIRGKAEGIGDYWYN
LIETNGHNVSLRSDPREMDADEVFDELSRADIRILDEIYSRYGHMNRFDLANMTHLESVCPEWHDPGNSRKPIDLKEMLI
SEGKSEDEANRIIGKMEESQKLKEFSLQLS

Nucleotide

• Length: 573 bp

>AT121537 NZ_CP038328:c5250472-5249900 [Escherichia coli O157:H7]
ATGTTTTGCGAAGAAAAAGTAGCTCAAATGGCTGCATACCTACTTCTTAAGAGAGGTGGGCGCATGGCATATCTGAAATT
GATGAAGCTGCTCTATCTGTCTAACCGCCAGTCGATTTTGAAGCATGGCAGGATGATCGGCGAAGATAGCCTTTACTCTA
TGAAATTTGGACCAGTCATGTCGAATACGCTGAACTTGATTCGCGGTAAGGCTGAAGGCATTGGTGACTACTGGTACAAC
TTGATAGAGACGAACGGGCATAATGTATCGTTGCGTTCAGATCCGAGGGAAATGGATGCAGACGAGGTCTTTGATGAATT
GAGTCGTGCAGATATCCGGATTTTAGATGAAATCTATTCTCGGTATGGGCATATGAACCGATTTGATCTCGCAAATATGA
CGCATTTAGAAAGCGTTTGTCCAGAGTGGCACGATCCTGGCAATTCTCGTAAGCCTATAGACCTGAAAGAAATGCTGATC
AGTGAGGGTAAAAGCGAGGATGAGGCTAATCGCATAATTGGCAAAATGGAAGAATCTCAGAAACTTAAGGAATTTTCTTT
GCAATTATCATGA

Structures

Antitoxin

• AlphaFold DB: A0A0J5Z6D5