Detailed information of TA system TA121524

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): paaR-paaA-parE/-
• Location: 2614018..2614489
• Replicon: chromosome
• Accession: NZ_CP038328
• Organism: Escherichia coli O157:H7 strain NE 1092-2

Toxin (Protein)

• Gene name: parE_1
• Uniprot ID: A0A0D7C2L1
• Locus tag: E4U73_RS13205
• Protein ID: WP_001303511.1
• Coordinates: 2614018..2614296 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: paaA
• Uniprot ID: Q8XAD5
• Locus tag: E4U73_RS13210
• Protein ID: WP_001302048.1
• Coordinates: 2614298..2614489 (-)
• Length: 64 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E4U73_RS13165	2609024..2609208	-	185	Protein_2574	DUF3596 domain-containing protein	-
E4U73_RS13170	2609243..2609494	-	252	WP_000005552.1	excisionase family protein	-
E4U73_RS13175	2609567..2612038	-	2472	WP_000048458.1	exonuclease	-
E4U73_RS13180	2612131..2612322	-	192	WP_001090200.1	DUF1482 family protein	-
E4U73_RS13185	2612319..2612507	-	189	WP_000449192.1	cell division inhibition protein DicB	-
E4U73_RS13190	2612908..2613072	+	165	WP_001331716.1	hypothetical protein	-
E4U73_RS13195	2613076..2613294	-	219	WP_001171930.1	hypothetical protein	-
E4U73_RS13200	2613366..2613665	-	300	WP_001240334.1	hypothetical protein	-
E4U73_RS13205	2614018..2614296	-	279	WP_001303511.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
E4U73_RS13210	2614298..2614489	-	192	WP_001302048.1	hypothetical protein	Antitoxin
E4U73_RS13215	2614510..2614881	-	372	WP_001169686.1	hypothetical protein	-
E4U73_RS13220	2614979..2615281	+	303	WP_000172738.1	transcriptional regulator	-
E4U73_RS13225	2615278..2615703	+	426	WP_000693943.1	Rha family transcriptional regulator	-
E4U73_RS13230	2615726..2616688	+	963	WP_000095668.1	helix-turn-helix domain-containing protein	-
E4U73_RS13235	2616695..2617435	+	741	WP_000788938.1	ATP-binding protein	-
E4U73_RS13240	2617461..2618230	+	770	Protein_2589	DUF1627 domain-containing protein	-
E4U73_RS13245	2618246..2618641	+	396	WP_001118159.1	DUF977 family protein	-
E4U73_RS13250	2618698..2619282	+	585	WP_000206793.1	DUF551 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2599138..2651702	52564

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10661.25 Da
• Isoelectric Point: 5.5647

>T121524 WP_001303511.1 NZ_CP038328:c2614296-2614018 [Escherichia coli O157:H7]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Nucleotide

• Length: 279 bp

>T121524 NZ_CP038328:c2614296-2614018 [Escherichia coli O157:H7]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin

Protein

• Length: 64 a.a.
• Molecular weight: 7463.47 Da
• Isoelectric Point: 8.6828

>AT121524 WP_001302048.1 NZ_CP038328:c2614489-2614298 [Escherichia coli O157:H7]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Nucleotide

• Length: 192 bp

>AT121524 NZ_CP038328:c2614489-2614298 [Escherichia coli O157:H7]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Structures

Toxin

• PDB: 5CZF
• PDB: 5CW7
• PDB: 5CZE

Antitoxin

• PDB: 5CZF
• PDB: 3ZBE
• PDB: 5CW7
• AlphaFold DB: A0A829CTP0