121508

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 377836..378467 Replicon chromosome
Accession NZ_CP038328
Organism Escherichia coli O157:H7 strain NE 1092-2

Toxin (Protein)


Gene name hicA Uniprot ID Q8X5S6
Locus tag E4U73_RS01760 Protein ID WP_001259386.1
Coordinates 377836..378111 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag E4U73_RS01765 Protein ID WP_000593555.1
Coordinates 378108..378467 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E4U73_RS01745 372840..373907 + 1068 WP_000361458.1 HlyD family secretion protein -
E4U73_RS01750 373904..376639 + 2736 WP_000149107.1 ribosome-associated ATPase/putative transporter RbbA -
E4U73_RS01755 376639..377763 + 1125 WP_001301659.1 ABC transporter permease -
E4U73_RS01760 377836..378111 + 276 WP_001259386.1 type II toxin-antitoxin system HicA family toxin Toxin
E4U73_RS01765 378108..378467 + 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
E4U73_RS01770 378517..379377 - 861 WP_001131788.1 class II aldolase -
E4U73_RS01775 379409..379678 - 270 WP_000933060.1 HPr family phosphocarrier protein -
E4U73_RS01780 379668..381176 - 1509 WP_001301773.1 carbohydrate kinase -
E4U73_RS01785 381169..382527 - 1359 WP_001302220.1 PTS galactitol transporter subunit IIC -
E4U73_RS01790 382604..382885 - 282 WP_000084025.1 PTS sugar transporter subunit IIB -
E4U73_RS01795 382882..383355 - 474 WP_001161553.1 PTS sugar transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 368980..378467 9487


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10165.92 Da        Isoelectric Point: 10.7076

>T121508 WP_001259386.1 NZ_CP038328:377836-378111 [Escherichia coli O157:H7]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKLILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T121508 NZ_CP038328:377836-378111 [Escherichia coli O157:H7]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGC
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT121508 WP_000593555.1 NZ_CP038328:378108-378467 [Escherichia coli O157:H7]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT121508 NZ_CP038328:378108-378467 [Escherichia coli O157:H7]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1I9WXY7

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References