Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4226043..4226661 Replicon chromosome
Accession NZ_CP038324
Organism Escherichia coli O157:H7 strain NE 1169-1

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag E4U74_RS21250 Protein ID WP_001291435.1
Coordinates 4226443..4226661 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag E4U74_RS21245 Protein ID WP_000344800.1
Coordinates 4226043..4226417 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E4U74_RS21235 4221132..4222325 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
E4U74_RS21240 4222348..4225497 + 3150 WP_001132452.1 multidrug efflux RND transporter permease subunit -
E4U74_RS21245 4226043..4226417 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
E4U74_RS21250 4226443..4226661 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
E4U74_RS21255 4226833..4227384 + 552 WP_000102553.1 maltose O-acetyltransferase -
E4U74_RS21260 4227500..4227970 + 471 WP_000136192.1 YlaC family protein -
E4U74_RS21265 4228134..4229684 + 1551 WP_001301851.1 cyclic-guanylate-specific phosphodiesterase PdeB -
E4U74_RS21270 4229726..4230079 - 354 WP_000878140.1 DUF1428 domain-containing protein -
E4U74_RS21280 4230458..4230769 + 312 WP_000409911.1 MGMT family protein -
E4U74_RS21285 4230802..4231374 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T121497 WP_001291435.1 NZ_CP038324:4226443-4226661 [Escherichia coli O157:H7]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T121497 NZ_CP038324:4226443-4226661 [Escherichia coli O157:H7]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT121497 WP_000344800.1 NZ_CP038324:4226043-4226417 [Escherichia coli O157:H7]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT121497 NZ_CP038324:4226043-4226417 [Escherichia coli O157:H7]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACTCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References