Detailed information of TA system TA121494

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3659509..3660307
• Replicon: chromosome
• Accession: NZ_CP038324
• Organism: Escherichia coli O157:H7 strain NE 1169-1

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A0V7WHV8
• Locus tag: E4U74_RS18730
• Protein ID: WP_001506713.1
• Coordinates: 3659509..3659886 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A093DMQ4
• Locus tag: E4U74_RS18735
• Protein ID: WP_001280952.1
• Coordinates: 3659933..3660307 (-)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E4U74_RS18700	3656190..3656894	+	705	WP_001241680.1	leucyl/phenylalanyl-tRNA--protein transferase	-
E4U74_RS18705	3657179..3657397	+	219	WP_001040187.1	translation initiation factor IF-1	-
E4U74_RS18715	3657888..3658730	-	843	WP_001688445.1	DUF4942 domain-containing protein	-
E4U74_RS18720	3658815..3659012	-	198	WP_000839282.1	DUF957 domain-containing protein	-
E4U74_RS18725	3659024..3659512	-	489	WP_001470575.1	hypothetical protein	-
E4U74_RS18730	3659509..3659886	-	378	WP_001506713.1	TA system toxin CbtA family protein	Toxin
E4U74_RS18735	3659933..3660307	-	375	WP_001280952.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
E4U74_RS18740	3660470..3660691	-	222	WP_001220316.1	DUF987 domain-containing protein	-
E4U74_RS18745	3660760..3661236	-	477	WP_001186714.1	RadC family protein	-
E4U74_RS18750	3661252..3661737	-	486	WP_001470565.1	antirestriction protein	-
E4U74_RS18755	3661829..3662647	-	819	WP_001506712.1	DUF945 domain-containing protein	-
E4U74_RS18760	3662987..3664057	-	1071	WP_000102643.1	patatin-like phospholipase family protein	-
E4U74_RS18765	3664054..3664959	-	906	WP_021497799.1	yjcZ-like family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-118)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14178.26 Da
• Isoelectric Point: 7.8045

>T121494 WP_001506713.1 NZ_CP038324:c3659886-3659509 [Escherichia coli O157:H7]
MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYITVNNITLGKHPEAKR

Nucleotide

• Length: 378 bp

>T121494 NZ_CP038324:c3659886-3659509 [Escherichia coli O157:H7]
ATGAAAACATTATCCGACACACATGTACGGGAGGTATCTCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAT
CCGACTGCTGGACCAGCATTATGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGTATTTCACTGTGTGATGCGGTGAACTTTCTCGTTGAAAAATACGCCCTGGTACGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCCGGGCAACAGGCCTGATGACCCGCGA
CAATTACATAACGGTAAATAACATTACCCTGGGGAAGCATCCGGAGGCAAAACGATGA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 13719.50 Da
• Isoelectric Point: 6.6249

>AT121494 WP_001280952.1 NZ_CP038324:c3660307-3659933 [Escherichia coli O157:H7]
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRNQHTVTLYAKGLTCEADTLGSCGYVYLAVYPAQAPATTS

Nucleotide

• Length: 375 bp

>AT121494 NZ_CP038324:c3660307-3659933 [Escherichia coli O157:H7]
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGTGCCCGTCTGGTGCAGGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAGCTCATGCTCACCAGCGGT
GAACTGAATCCCCGAAATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGGCACAGGCACCCGCAACCACCTCATAA

Structures

Toxin

• AlphaFold DB: A0A0V7WHV8

Antitoxin

• AlphaFold DB: A0A093DMQ4