121439

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 31132..31657 Replicon plasmid pNE122-1
Accession NZ_CP038320
Organism Escherichia coli O157:H7 strain NE122

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag E3167_RS26980 Protein ID WP_001159868.1
Coordinates 31352..31657 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag E3167_RS26975 Protein ID WP_000813634.1
Coordinates 31132..31350 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E3167_RS26945 26415..26645 + 231 WP_010891288.1 hypothetical protein -
E3167_RS26950 26766..27506 + 741 WP_001066920.1 tyrosine-type recombinase/integrase -
E3167_RS26955 27791..28768 - 978 WP_000361615.1 RepB family plasmid replication initiator protein -
E3167_RS26960 29176..29376 - 201 WP_000708307.1 hypothetical protein -
E3167_RS26965 29373..29993 - 621 WP_001248529.1 hypothetical protein -
E3167_RS26970 29990..30673 - 684 WP_010891291.1 DNA-binding protein -
E3167_RS26975 31132..31350 + 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
E3167_RS26980 31352..31657 + 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
E3167_RS26985 31658..32464 + 807 WP_000016989.1 site-specific integrase -
E3167_RS26995 34454..34816 + 363 Protein_34 RepB family plasmid replication initiator protein -
E3167_RS27000 35404..36570 + 1167 WP_000772446.1 plasmid-partitioning protein SopA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - stcE / exeE / exeG / hlyC / hlyA / hlyB / hlyD / toxB / espP 1..95338 95338


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T121439 WP_001159868.1 NZ_CP038320:31352-31657 [Escherichia coli O157:H7]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T121439 NZ_CP038320:31352-31657 [Escherichia coli O157:H7]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT121439 WP_000813634.1 NZ_CP038320:31132-31350 [Escherichia coli O157:H7]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT121439 NZ_CP038320:31132-31350 [Escherichia coli O157:H7]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
AlphaFold DB A0A829CQY2

References