Detailed information of TA system TA121429

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): paaR-paaA-parE/-
• Location: 3503280..3503758
• Replicon: chromosome
• Accession: NZ_CP038319
• Organism: Escherichia coli O157:H7 strain NE122

Toxin (Protein)

• Gene name: parE_1
• Uniprot ID: A0A891SFN9
• Locus tag: E3167_RS17815
• Protein ID: WP_001303876.1
• Coordinates: 3503471..3503758 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: paaA
• Uniprot ID: Q8XD67
• Locus tag: E3167_RS17810
• Protein ID: WP_000536233.1
• Coordinates: 3503280..3503471 (+)
• Length: 64 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3167_RS17775	3498690..3499460	-	771	WP_000450992.1	DUF1627 domain-containing protein	-
E3167_RS17780	3499482..3500228	-	747	WP_000788745.1	ATP-binding protein	-
E3167_RS17785	3500235..3501320	-	1086	WP_072141648.1	DNA-binding protein	-
E3167_RS17790	3501399..3501854	+	456	WP_000273724.1	hypothetical protein	-
E3167_RS17795	3502061..3502486	-	426	WP_000693855.1	Rha family transcriptional regulator	-
E3167_RS17800	3502470..3502742	-	273	WP_000887453.1	helix-turn-helix domain-containing protein	-
E3167_RS17805	3502851..3503252	+	402	WP_000986592.1	helix-turn-helix domain-containing protein	-
E3167_RS17810	3503280..3503471	+	192	WP_000536233.1	hypothetical protein	Antitoxin
E3167_RS17815	3503471..3503758	+	288	WP_001303876.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
E3167_RS17820	3504036..3504191	+	156	WP_000379575.1	DUF1391 family protein	-
E3167_RS17825	3504333..3504722	+	390	WP_000394511.1	hypothetical protein	-
E3167_RS17830	3504909..3505094	-	186	WP_001133046.1	hypothetical protein	-
E3167_RS17835	3505668..3505856	+	189	WP_000413705.1	cell division inhibition protein DicB	-
E3167_RS17840	3505853..3506044	+	192	WP_001098307.1	DUF1482 family protein	-
E3167_RS17845	3506138..3508609	+	2472	WP_000034474.1	exonuclease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 10978.84 Da
• Isoelectric Point: 10.1360

>T121429 WP_001303876.1 NZ_CP038319:3503471-3503758 [Escherichia coli O157:H7]
MLPILWLPSARDDLRQIITYIAKENPPAARRLKIRIETSVLPLSEHPYLYPPSERVSGLREIVTHPNYIILYRVAASSIE
IVSVTHSRRQFPFSI

Nucleotide

• Length: 288 bp

>T121429 NZ_CP038319:3503471-3503758 [Escherichia coli O157:H7]
ATGTTACCCATTTTATGGCTACCGTCTGCTCGCGATGATTTGCGCCAGATCATAACTTACATCGCCAAGGAGAACCCACC
GGCAGCACGTAGACTAAAAATACGCATTGAAACATCGGTATTACCTCTATCTGAGCATCCGTACTTATATCCACCAAGCG
AACGGGTTTCTGGATTGAGAGAGATCGTGACCCACCCTAACTACATAATCCTGTACAGAGTAGCTGCTTCAAGCATTGAG
ATTGTAAGCGTGACACATTCTCGGCGACAATTTCCCTTCTCTATCTGA

Antitoxin

Protein

• Length: 64 a.a.
• Molecular weight: 7025.80 Da
• Isoelectric Point: 4.0270

>AT121429 WP_000536233.1 NZ_CP038319:3503280-3503471 [Escherichia coli O157:H7]
MGTALSPIVSEFETTEQENSYNEWLRTKVTSSLADTRPAIPHDEVMAEMENLIAQIAVTNKSE

Nucleotide

• Length: 192 bp

>AT121429 NZ_CP038319:3503280-3503471 [Escherichia coli O157:H7]
ATGGGTACAGCCCTTTCTCCGATAGTTTCAGAATTCGAAACTACCGAACAAGAAAACAGTTACAACGAATGGTTGCGCAC
TAAAGTAACGTCAAGCCTTGCAGACACTCGCCCCGCAATTCCACATGACGAGGTAATGGCTGAAATGGAAAATCTTATTG
CTCAAATTGCTGTAACTAACAAGAGCGAGTAA