Detailed information of TA system TA121424

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2743502..2744140
• Replicon: chromosome
• Accession: NZ_CP038319
• Organism: Escherichia coli O157:H7 strain NE122

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: E3167_RS13795
• Protein ID: WP_000813794.1
• Coordinates: 2743964..2744140 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: E3167_RS13790
• Protein ID: WP_001270286.1
• Coordinates: 2743502..2743918 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3167_RS13770	2738654..2739595	-	942	WP_171877086.1	ABC transporter permease subunit	-
E3167_RS13775	2739596..2740609	-	1014	WP_000220402.1	ABC transporter ATP-binding protein	-
E3167_RS13780	2740627..2741772	-	1146	WP_000047432.1	ABC transporter substrate-binding protein	-
E3167_RS13785	2742017..2743423	-	1407	WP_000760654.1	PLP-dependent aminotransferase family protein	-
E3167_RS13790	2743502..2743918	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
E3167_RS13795	2743964..2744140	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
E3167_RS13800	2744362..2744592	+	231	WP_000494244.1	YncJ family protein	-
E3167_RS13805	2744684..2746645	-	1962	WP_001303492.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
E3167_RS13810	2746718..2747254	-	537	WP_000429145.1	DNA-binding transcriptional regulator SutR	-
E3167_RS13815	2747346..2748519	+	1174	Protein_2699	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T121424 WP_000813794.1 NZ_CP038319:c2744140-2743964 [Escherichia coli O157:H7]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T121424 NZ_CP038319:c2744140-2743964 [Escherichia coli O157:H7]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT121424 WP_001270286.1 NZ_CP038319:c2743918-2743502 [Escherichia coli O157:H7]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT121424 NZ_CP038319:c2743918-2743502 [Escherichia coli O157:H7]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAAGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• PDB: 6HPB