Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4100787..4101405 Replicon chromosome
Accession NZ_CP038316
Organism Escherichia coli O157:H7 strain NE92

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag E3166_RS20485 Protein ID WP_001291435.1
Coordinates 4101187..4101405 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag E3166_RS20480 Protein ID WP_000344800.1
Coordinates 4100787..4101161 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E3166_RS20470 4095876..4097069 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
E3166_RS20475 4097092..4100241 + 3150 WP_001132452.1 multidrug efflux RND transporter permease subunit -
E3166_RS20480 4100787..4101161 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
E3166_RS20485 4101187..4101405 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
E3166_RS20490 4101577..4102128 + 552 WP_000102553.1 maltose O-acetyltransferase -
E3166_RS20495 4102244..4102714 + 471 WP_000136192.1 YlaC family protein -
E3166_RS20500 4102878..4104428 + 1551 WP_001301851.1 cyclic-guanylate-specific phosphodiesterase PdeB -
E3166_RS20505 4104470..4104823 - 354 WP_000878140.1 DUF1428 domain-containing protein -
E3166_RS20515 4105202..4105513 + 312 WP_000409911.1 MGMT family protein -
E3166_RS20520 4105546..4106118 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T121393 WP_001291435.1 NZ_CP038316:4101187-4101405 [Escherichia coli O157:H7]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T121393 NZ_CP038316:4101187-4101405 [Escherichia coli O157:H7]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT121393 WP_000344800.1 NZ_CP038316:4100787-4101161 [Escherichia coli O157:H7]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT121393 NZ_CP038316:4100787-4101161 [Escherichia coli O157:H7]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACTCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References