Detailed information of TA system TA121384

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): paaR-paaA-parE/-
• Location: 2505644..2506115
• Replicon: chromosome
• Accession: NZ_CP038316
• Organism: Escherichia coli O157:H7 strain NE92

Toxin (Protein)

• Gene name: parE_1
• Uniprot ID: A0A0D7C2L1
• Locus tag: E3166_RS12400
• Protein ID: WP_001303511.1
• Coordinates: 2505644..2505922 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: paaA
• Uniprot ID: Q8XAD5
• Locus tag: E3166_RS12405
• Protein ID: WP_001302048.1
• Coordinates: 2505924..2506115 (-)
• Length: 64 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3166_RS12360	2500653..2500837	-	185	Protein_2414	DUF3596 domain-containing protein	-
E3166_RS12365	2500872..2501123	-	252	WP_000005552.1	excisionase family protein	-
E3166_RS12370	2501196..2503667	-	2472	WP_171889491.1	3'-5' exoribonuclease	-
E3166_RS12375	2503760..2503951	-	192	WP_001090200.1	DUF1482 family protein	-
E3166_RS12380	2503948..2504136	-	189	WP_000449192.1	cell division inhibition protein DicB	-
E3166_RS12385	2504534..2504698	+	165	WP_001331716.1	hypothetical protein	-
E3166_RS12390	2504702..2504920	-	219	WP_001171930.1	hypothetical protein	-
E3166_RS12395	2504992..2505291	-	300	WP_001240334.1	hypothetical protein	-
E3166_RS12400	2505644..2505922	-	279	WP_001303511.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
E3166_RS12405	2505924..2506115	-	192	WP_001302048.1	hypothetical protein	Antitoxin
E3166_RS12410	2506136..2506507	-	372	WP_001169686.1	hypothetical protein	-
E3166_RS12415	2506605..2506907	+	303	WP_000172738.1	transcriptional regulator	-
E3166_RS12420	2506904..2507329	+	426	WP_000693943.1	Rha family transcriptional regulator	-
E3166_RS12425	2507352..2508314	+	963	WP_000095669.1	helix-turn-helix domain-containing protein	-
E3166_RS12430	2508321..2509061	+	741	WP_000788938.1	ATP-binding protein	-
E3166_RS12435	2509087..2509856	+	770	Protein_2429	DUF1627 domain-containing protein	-
E3166_RS12440	2509872..2510267	+	396	WP_001118159.1	DUF977 family protein	-
E3166_RS12445	2510324..2510908	+	585	WP_000206793.1	DUF551 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	nleG7' / nleA/espI	2490767..2596820	106053

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10661.25 Da
• Isoelectric Point: 5.5647

>T121384 WP_001303511.1 NZ_CP038316:c2505922-2505644 [Escherichia coli O157:H7]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Nucleotide

• Length: 279 bp

>T121384 NZ_CP038316:c2505922-2505644 [Escherichia coli O157:H7]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin

Protein

• Length: 64 a.a.
• Molecular weight: 7463.47 Da
• Isoelectric Point: 8.6828

>AT121384 WP_001302048.1 NZ_CP038316:c2506115-2505924 [Escherichia coli O157:H7]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Nucleotide

• Length: 192 bp

>AT121384 NZ_CP038316:c2506115-2505924 [Escherichia coli O157:H7]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Structures

Toxin

• PDB: 5CZF
• PDB: 5CW7
• PDB: 5CZE

Antitoxin

• PDB: 5CZF
• PDB: 3ZBE
• PDB: 5CW7
• AlphaFold DB: A0A829CTP0