Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 65214..65739 Replicon plasmid pOK1-1
Accession NZ_CP038314
Organism Escherichia coli O157:H7 strain OK1

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag E3173_RS27295 Protein ID WP_001159868.1
Coordinates 65214..65519 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag E3173_RS27300 Protein ID WP_000813634.1
Coordinates 65521..65739 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E3173_RS27275 60417..61583 - 1167 WP_000772446.1 plasmid-partitioning protein SopA -
E3173_RS27280 62171..62509 - 339 WP_071525396.1 RepB family plasmid replication initiator protein -
E3173_RS27285 62471..63684 + 1214 WP_162829202.1 IS3 family transposase -
E3173_RS27290 64407..65213 - 807 WP_000016989.1 site-specific integrase -
E3173_RS27295 65214..65519 - 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
E3173_RS27300 65521..65739 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
E3173_RS27305 66198..66881 + 684 WP_010891291.1 DNA-binding protein -
E3173_RS27310 66878..67498 + 621 WP_001248529.1 hypothetical protein -
E3173_RS27315 67495..67695 + 201 WP_000708307.1 hypothetical protein -
E3173_RS27320 68103..69080 + 978 WP_000361615.1 RepB family plasmid replication initiator protein -
E3173_RS27325 69365..70105 - 741 WP_001066920.1 tyrosine-type recombinase/integrase -
E3173_RS27330 70226..70456 - 231 WP_010891288.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - stcE / exeE / exeG / toxB / hlyD / hlyB / hlyA / hlyC / espP 1..97630 97630


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T121366 WP_001159868.1 NZ_CP038314:c65519-65214 [Escherichia coli O157:H7]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T121366 NZ_CP038314:c65519-65214 [Escherichia coli O157:H7]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT121366 WP_000813634.1 NZ_CP038314:c65739-65521 [Escherichia coli O157:H7]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT121366 NZ_CP038314:c65739-65521 [Escherichia coli O157:H7]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References