Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | relBE/RHH(antitoxin) |
| Location | 19521..20080 | Replicon | plasmid pOK1-1 |
| Accession | NZ_CP038314 | ||
| Organism | Escherichia coli O157:H7 strain OK1 | ||
Toxin (Protein)
| Gene name | relE | Uniprot ID | O82928 |
| Locus tag | E3173_RS27040 | Protein ID | WP_000421248.1 |
| Coordinates | 19521..19796 (-) | Length | 92 a.a. |
Antitoxin (Protein)
| Gene name | relB | Uniprot ID | C1J8K5 |
| Locus tag | E3173_RS27045 | Protein ID | WP_001178089.1 |
| Coordinates | 19796..20080 (-) | Length | 95 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| E3173_RS27005 | 15725..17263 | - | 1539 | WP_000998048.1 | IS66-like element ISEc8 family transposase | - |
| E3173_RS27010 | 17313..17660 | - | 348 | WP_000612591.1 | IS66 family insertion sequence element accessory protein TnpB | - |
| E3173_RS27015 | 17657..18037 | - | 381 | WP_001171554.1 | IS66 family insertion sequence hypothetical protein | - |
| E3173_RS27020 | 18131..18295 | + | 165 | Protein_18 | IS91 family transposase | - |
| E3173_RS27025 | 18295..18861 | + | 567 | Protein_19 | IS3-like element IS600 family transposase | - |
| E3173_RS27030 | 18905..19120 | + | 216 | Protein_20 | transposase | - |
| E3173_RS27035 | 19220..19426 | - | 207 | WP_001105064.1 | hypothetical protein | - |
| E3173_RS27040 | 19521..19796 | - | 276 | WP_000421248.1 | type II toxin-antitoxin system RelE/ParE family toxin | Toxin |
| E3173_RS27045 | 19796..20080 | - | 285 | WP_001178089.1 | ribbon-helix-helix domain-containing protein | Antitoxin |
| E3173_RS27050 | 20992..21849 | - | 858 | WP_000130945.1 | incFII family plasmid replication initiator RepA | - |
| E3173_RS27055 | 21842..21916 | - | 75 | WP_001370046.1 | RepA leader peptide Tap | - |
| E3173_RS27060 | 22152..22406 | - | 255 | WP_000083831.1 | replication regulatory protein RepA | - |
| E3173_RS27065 | 22646..22984 | - | 339 | WP_000766796.1 | hypothetical protein | - |
| E3173_RS27070 | 23022..23231 | - | 210 | WP_001302200.1 | hemolysin expression modulator Hha | - |
| E3173_RS27075 | 23277..23738 | - | 462 | WP_001233853.1 | thermonuclease family protein | - |
| E3173_RS27080 | 23983..24195 | - | 213 | WP_001302189.1 | ANR family transcriptional regulator | - |
| E3173_RS27085 | 24328..24888 | - | 561 | WP_000840472.1 | fertility inhibition protein FinO | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | - | stcE / exeE / exeG / toxB / hlyD / hlyB / hlyA / hlyC / espP | 1..97630 | 97630 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 92 a.a. Molecular weight: 10735.42 Da Isoelectric Point: 9.9324
>T121365 WP_000421248.1 NZ_CP038314:c19796-19521 [Escherichia coli O157:H7]
MELKWSSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIY
VLRLWHTRENR
MELKWSSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIY
VLRLWHTRENR
Download Length: 276 bp
>T121365 NZ_CP038314:c19796-19521 [Escherichia coli O157:H7]
ATGGAACTGAAGTGGAGCAGTAAAGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTAGCCAGTAAACCTGC
TGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGGGAACAGTTATTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTAATGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTAG
ATGGAACTGAAGTGGAGCAGTAAAGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTAGCCAGTAAACCTGC
TGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGGGAACAGTTATTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTAATGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTAG
Antitoxin
Download Length: 95 a.a. Molecular weight: 10474.89 Da Isoelectric Point: 5.6558
>AT121365 WP_001178089.1 NZ_CP038314:c20080-19796 [Escherichia coli O157:H7]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR
Download Length: 285 bp
>AT121365 NZ_CP038314:c20080-19796 [Escherichia coli O157:H7]
ATGCAAATGAAAAACAATACCGCACAAGCAACAAAAGTCATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGTTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATTGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA
ATGCAAATGAAAAACAATACCGCACAAGCAACAAAAGTCATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGTTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATTGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A2Z6FAZ0 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A731IHA8 |