Detailed information of TA system TA121354

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): paaR-paaA-parE/-
• Location: 3463131..3463609
• Replicon: chromosome
• Accession: NZ_CP038313
• Organism: Escherichia coli O157:H7 strain OK1

Toxin (Protein)

• Gene name: parE_1
• Uniprot ID: A0A891SFN9
• Locus tag: E3173_RS17735
• Protein ID: WP_001303876.1
• Coordinates: 3463322..3463609 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: paaA
• Uniprot ID: Q8XD67
• Locus tag: E3173_RS17730
• Protein ID: WP_000536233.1
• Coordinates: 3463131..3463322 (+)
• Length: 64 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3173_RS17700	3458772..3459857	-	1086	WP_072141648.1	DNA-binding protein	-
E3173_RS17705	3459936..3460391	+	456	WP_000273724.1	hypothetical protein	-
E3173_RS17710	3460792..3462005	+	1214	WP_162829202.1	IS3 family transposase	-
E3173_RS17715	3462044..3462337	-	294	Protein_3469	Rha family transcriptional regulator	-
E3173_RS17720	3462321..3462593	-	273	WP_000887453.1	helix-turn-helix domain-containing protein	-
E3173_RS17725	3462702..3463103	+	402	WP_000986592.1	helix-turn-helix domain-containing protein	-
E3173_RS17730	3463131..3463322	+	192	WP_000536233.1	hypothetical protein	Antitoxin
E3173_RS17735	3463322..3463609	+	288	WP_001303876.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
E3173_RS17740	3463887..3464042	+	156	WP_000379575.1	DUF1391 family protein	-
E3173_RS17745	3464184..3464573	+	390	WP_000394511.1	hypothetical protein	-
E3173_RS17750	3464760..3464945	-	186	WP_001133046.1	hypothetical protein	-
E3173_RS17755	3465519..3465707	+	189	WP_000413705.1	cell division inhibition protein DicB	-
E3173_RS17760	3465704..3465895	+	192	WP_001098307.1	DUF1482 family protein	-
E3173_RS17765	3465989..3468460	+	2472	WP_000034474.1	exonuclease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	ompA	3461118..3491629	30511

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 10978.84 Da
• Isoelectric Point: 10.1360

>T121354 WP_001303876.1 NZ_CP038313:3463322-3463609 [Escherichia coli O157:H7]
MLPILWLPSARDDLRQIITYIAKENPPAARRLKIRIETSVLPLSEHPYLYPPSERVSGLREIVTHPNYIILYRVAASSIE
IVSVTHSRRQFPFSI

Nucleotide

• Length: 288 bp

>T121354 NZ_CP038313:3463322-3463609 [Escherichia coli O157:H7]
ATGTTACCCATTTTATGGCTACCGTCTGCTCGCGATGATTTGCGCCAGATCATAACTTACATCGCCAAGGAGAACCCACC
GGCAGCACGTAGACTAAAAATACGCATTGAAACATCGGTATTACCTCTATCTGAGCATCCGTACTTATATCCACCAAGCG
AACGGGTTTCTGGATTGAGAGAGATCGTGACCCACCCTAACTACATAATCCTGTACAGAGTAGCTGCTTCAAGCATTGAG
ATTGTAAGCGTGACACATTCTCGGCGACAATTTCCCTTCTCTATCTGA

Antitoxin

Protein

• Length: 64 a.a.
• Molecular weight: 7025.80 Da
• Isoelectric Point: 4.0270

>AT121354 WP_000536233.1 NZ_CP038313:3463131-3463322 [Escherichia coli O157:H7]
MGTALSPIVSEFETTEQENSYNEWLRTKVTSSLADTRPAIPHDEVMAEMENLIAQIAVTNKSE

Nucleotide

• Length: 192 bp

>AT121354 NZ_CP038313:3463131-3463322 [Escherichia coli O157:H7]
ATGGGTACAGCCCTTTCTCCGATAGTTTCAGAATTCGAAACTACCGAACAAGAAAACAGTTACAACGAATGGTTGCGCAC
TAAAGTAACGTCAAGCCTTGCAGACACTCGCCCCGCAATTCCACATGACGAGGTAATGGCTGAAATGGAAAATCTTATTG
CTCAAATTGCTGTAACTAACAAGAGCGAGTAA