Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 35031..35675 Replicon plasmid pKS470-3
Accession NZ_CP038311
Organism Escherichia coli O157:H7 strain Show KS 470-1

Toxin (Protein)


Gene name hicA Uniprot ID A0A7L6LII1
Locus tag E4U84_RS28255 Protein ID WP_000833471.1
Coordinates 35031..35213 (+) Length 61 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A7L6LHN8
Locus tag E4U84_RS28260 Protein ID WP_000466317.1
Coordinates 35238..35675 (+) Length 146 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E4U84_RS28215 30589..30912 + 324 WP_000432877.1 hypothetical protein -
E4U84_RS28220 30953..31177 + 225 WP_000866040.1 hypothetical protein -
E4U84_RS28225 31273..31497 + 225 WP_000749828.1 hypothetical protein -
E4U84_RS28230 31548..31814 + 267 WP_000702249.1 hypothetical protein -
E4U84_RS28235 31804..32046 + 243 WP_001450803.1 hypothetical protein -
E4U84_RS28240 32430..33617 - 1188 WP_171878221.1 IS91 family transposase -
E4U84_RS28245 33617..33982 - 366 WP_000091308.1 hypothetical protein -
E4U84_RS28250 34314..34592 + 279 WP_000545936.1 hypothetical protein -
E4U84_RS28255 35031..35213 + 183 WP_000833471.1 type II toxin-antitoxin system HicA family toxin Toxin
E4U84_RS28260 35238..35675 + 438 WP_000466317.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
E4U84_RS28265 35704..36408 - 705 WP_001279866.1 hypothetical protein -
E4U84_RS28270 36405..37166 - 762 WP_001097937.1 conjugal transfer protein TraL -
E4U84_RS28275 37176..37583 - 408 WP_001190619.1 hypothetical protein -
E4U84_RS28280 37929..38267 + 339 WP_000753495.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - - 1..43127 43127
- inside IScluster/Tn - - 25409..33617 8208


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(4-60)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(7-138)


Sequences

Toxin        


Download         Length: 61 a.a.        Molecular weight: 6675.97 Da        Isoelectric Point: 11.0013

>T121333 WP_000833471.1 NZ_CP038311:35031-35213 [Escherichia coli O157:H7]
MKSADLLKELIAAGCELKRHKASSHQIWWSPITGKTFPVPHPKKDLPLGTVRSIRKMAGI

Download         Length: 183 bp

>T121333 NZ_CP038311:35031-35213 [Escherichia coli O157:H7]
ATGAAATCTGCTGACCTGTTGAAAGAACTGATAGCTGCTGGTTGTGAGTTGAAGCGGCATAAGGCGAGCAGCCACCAGAT
ATGGTGGTCGCCCATAACAGGAAAAACTTTTCCTGTGCCCCATCCGAAAAAAGACTTGCCACTGGGTACTGTCAGATCGA
TCAGAAAAATGGCGGGGATTTAA

Antitoxin


Download         Length: 146 a.a.        Molecular weight: 16227.10 Da        Isoelectric Point: 4.3135

>AT121333 WP_000466317.1 NZ_CP038311:35238-35675 [Escherichia coli O157:H7]
MFFSVGVETPKDDHTAYGITVPAFDRFDFGCVSAADTQSEIPVMAREAILAIVEEMVLSGSYSVDDIHDDGCLTYAANQD
YSHCDSWFVIDVDLSEIEGKQQRINIALPDVLIRRIDGFVRESGGVYRDRSHFLAQAARHELSYK

Download         Length: 438 bp

>AT121333 NZ_CP038311:35238-35675 [Escherichia coli O157:H7]
ATGTTTTTTTCTGTGGGTGTCGAAACACCGAAAGATGACCATACGGCATATGGTATAACCGTTCCGGCTTTTGATCGTTT
TGATTTTGGCTGTGTCTCTGCTGCCGATACTCAATCAGAAATTCCTGTGATGGCGCGAGAAGCGATATTGGCTATCGTGG
AAGAAATGGTTTTAAGTGGCTCTTATTCTGTTGACGATATTCATGATGACGGCTGTTTAACATATGCCGCAAATCAGGAC
TATAGTCACTGCGATAGCTGGTTTGTAATTGATGTTGATTTGTCTGAAATTGAAGGTAAACAACAACGTATTAATATTGC
GTTGCCTGATGTGTTGATTCGTCGTATTGATGGATTTGTTAGAGAAAGTGGCGGTGTTTACAGAGATCGTAGCCATTTTC
TTGCTCAGGCCGCGCGGCATGAACTTTCATATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7L6LII1

Antitoxin

Source ID Structure
AlphaFold DB A0A7L6LHN8

References