Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) TsxAB/Xre(antitoxin)
Location 31548..32046 Replicon plasmid pKS470-3
Accession NZ_CP038311
Organism Escherichia coli O157:H7 strain Show KS 470-1

Toxin (Protein)


Gene name TsxA Uniprot ID A0A0K6A0X9
Locus tag E4U84_RS28230 Protein ID WP_000702249.1
Coordinates 31548..31814 (+) Length 89 a.a.

Antitoxin (Protein)


Gene name TsxB Uniprot ID A0A3H7VMJ5
Locus tag E4U84_RS28235 Protein ID WP_001450803.1
Coordinates 31804..32046 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E4U84_RS28210 27905..28909 - 1005 WP_001208090.1 replication initiation protein -
E4U84_RS28215 30589..30912 + 324 WP_000432877.1 hypothetical protein -
E4U84_RS28220 30953..31177 + 225 WP_000866040.1 hypothetical protein -
E4U84_RS28225 31273..31497 + 225 WP_000749828.1 hypothetical protein -
E4U84_RS28230 31548..31814 + 267 WP_000702249.1 hypothetical protein Toxin
E4U84_RS28235 31804..32046 + 243 WP_001450803.1 hypothetical protein Antitoxin
E4U84_RS28240 32430..33617 - 1188 WP_171878221.1 IS91 family transposase -
E4U84_RS28245 33617..33982 - 366 WP_000091308.1 hypothetical protein -
E4U84_RS28250 34314..34592 + 279 WP_000545936.1 hypothetical protein -
E4U84_RS28255 35031..35213 + 183 WP_000833471.1 type II toxin-antitoxin system HicA family toxin -
E4U84_RS28260 35238..35675 + 438 WP_000466317.1 type II toxin-antitoxin system HicB family antitoxin -
E4U84_RS28265 35704..36408 - 705 WP_001279866.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - - 1..43127 43127
- inside IScluster/Tn - - 25409..33617 8208


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-132)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 89 a.a.        Molecular weight: 10242.76 Da        Isoelectric Point: 9.9280

>T121332 WP_000702249.1 NZ_CP038311:31548-31814 [Escherichia coli O157:H7]
MKIRKGDRQYYLNKEGNMFHLVKRVKTFSKSATLGKTKATVITVADLIFHEDAFDTIDFTRGGLRENDKEVVSMMIQEMN
NEREKNVN

Download         Length: 267 bp

>T121332 NZ_CP038311:31548-31814 [Escherichia coli O157:H7]
ATGAAGATACGAAAAGGTGACAGGCAATATTATTTGAATAAAGAAGGTAATATGTTTCATCTTGTGAAACGGGTGAAGAC
CTTTTCTAAATCTGCGACTTTAGGGAAAACAAAAGCGACAGTGATAACTGTTGCCGATCTAATTTTTCATGAAGATGCAT
TCGACACTATCGACTTTACCAGAGGCGGGTTAAGAGAGAACGACAAAGAAGTTGTTTCTATGATGATTCAGGAAATGAAC
AATGAACGAGAAAAAAATGTTAACTGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9288.69 Da        Isoelectric Point: 9.7120

>AT121332 WP_001450803.1 NZ_CP038311:31804-32046 [Escherichia coli O157:H7]
MLTENTPENIKRLRQKIGLTQRECAEIFSMNPRTWRRKEEPAGTASGTALTPVEFKYLQLLAGEHPEYVLRKRDKPKCSE

Download         Length: 243 bp

>AT121332 NZ_CP038311:31804-32046 [Escherichia coli O157:H7]
ATGTTAACTGAAAATACACCAGAAAACATTAAACGTCTGCGGCAAAAAATTGGCCTGACGCAAAGAGAATGTGCTGAAAT
ATTCAGCATGAACCCGCGAACCTGGCGTCGCAAGGAAGAACCTGCCGGAACTGCAAGCGGTACAGCACTTACACCAGTAG
AGTTTAAATATCTTCAGCTTTTAGCTGGGGAACATCCTGAATACGTCCTGCGCAAAAGGGATAAACCTAAATGTAGTGAA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0K6A0X9

Antitoxin

Source ID Structure
AlphaFold DB A0A3H7VMJ5

References