121290

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 31734..32378 Replicon plasmid pNE1040-3
Accession NZ_CP038307
Organism Escherichia coli O157:H7 strain SS NE 1040-1

Toxin (Protein)


Gene name hicA Uniprot ID A0A7L6LII1
Locus tag E4U78_RS27540 Protein ID WP_000833471.1
Coordinates 31734..31916 (+) Length 61 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A7L6LHN8
Locus tag E4U78_RS27545 Protein ID WP_000466317.1
Coordinates 31941..32378 (+) Length 146 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E4U78_RS27505 28741..29064 + 324 WP_000432877.1 hypothetical protein -
E4U78_RS27510 29104..29328 + 225 WP_000866040.1 hypothetical protein -
E4U78_RS27515 29424..29648 + 225 WP_000749828.1 hypothetical protein -
E4U78_RS27520 29699..29965 + 267 WP_000868277.1 hypothetical protein -
E4U78_RS27525 29955..30197 + 243 WP_001479502.1 transcriptional regulator -
E4U78_RS27530 30636..30965 + 330 WP_000855123.1 hypothetical protein -
E4U78_RS27535 31023..31301 + 279 WP_000545933.1 hypothetical protein -
E4U78_RS27540 31734..31916 + 183 WP_000833471.1 type II toxin-antitoxin system HicA family toxin Toxin
E4U78_RS27545 31941..32378 + 438 WP_000466317.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
E4U78_RS27550 32407..33111 - 705 WP_001279862.1 hypothetical protein -
E4U78_RS27555 33108..33869 - 762 WP_001097938.1 conjugal transfer protein TraL -
E4U78_RS27560 33879..34286 - 408 WP_001190621.1 hypothetical protein -
E4U78_RS27565 34633..34968 + 336 WP_000963832.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..38515 38515


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(4-60)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(7-138)


Sequences

Toxin        


Download         Length: 61 a.a.        Molecular weight: 6675.97 Da        Isoelectric Point: 11.0013

>T121290 WP_000833471.1 NZ_CP038307:31734-31916 [Escherichia coli O157:H7]
MKSADLLKELIAAGCELKRHKASSHQIWWSPITGKTFPVPHPKKDLPLGTVRSIRKMAGI

Download         Length: 183 bp

>T121290 NZ_CP038307:31734-31916 [Escherichia coli O157:H7]
ATGAAATCTGCTGACCTGTTGAAAGAACTGATAGCTGCTGGTTGTGAGTTGAAGCGGCATAAGGCGAGCAGCCACCAGAT
ATGGTGGTCGCCCATAACAGGAAAAACTTTTCCTGTGCCCCATCCGAAAAAAGACTTGCCACTGGGTACTGTCAGATCGA
TCAGAAAAATGGCGGGGATTTAA

Antitoxin


Download         Length: 146 a.a.        Molecular weight: 16227.10 Da        Isoelectric Point: 4.3135

>AT121290 WP_000466317.1 NZ_CP038307:31941-32378 [Escherichia coli O157:H7]
MFFSVGVETPKDDHTAYGITVPAFDRFDFGCVSAADTQSEIPVMAREAILAIVEEMVLSGSYSVDDIHDDGCLTYAANQD
YSHCDSWFVIDVDLSEIEGKQQRINIALPDVLIRRIDGFVRESGGVYRDRSHFLAQAARHELSYK

Download         Length: 438 bp

>AT121290 NZ_CP038307:31941-32378 [Escherichia coli O157:H7]
ATGTTTTTTTCTGTGGGTGTCGAAACACCGAAAGATGACCATACGGCATATGGTATAACCGTTCCGGCTTTTGATCGTTT
TGATTTTGGCTGTGTCTCTGCTGCCGATACTCAATCAGAAATTCCTGTGATGGCGCGAGAAGCGATATTGGCTATCGTGG
AAGAAATGGTTTTAAGTGGTTCTTATTCTGTTGATGATATTCATGATGACGGCTGTTTAACATATGCCGCAAATCAGGAC
TATAGTCACTGCGATAGCTGGTTTGTAATTGATGTTGATTTGTCTGAAATTGAAGGTAAACAACAACGTATTAATATTGC
GTTGCCTGATGTGTTGATTCGTCGTATTGATGGATTTGTCAGAGAAAGTGGCGGTGTTTACAGAGATCGTAGCCATTTTC
TTGCTCAGGCCGCGCGGCATGAACTTTCATATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7L6LII1

Antitoxin

Source ID Structure
AlphaFold DB A0A7L6LHN8

References