Detailed information of TA system TA121289

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsxAB/Xre(antitoxin)
• Location: 29699..30197
• Replicon: plasmid pNE1040-3
• Accession: NZ_CP038307
• Organism: Escherichia coli O157:H7 strain SS NE 1040-1

Toxin (Protein)

• Gene name: TsxA
• Uniprot ID: A0A7L6LHZ8
• Locus tag: E4U78_RS27520
• Protein ID: WP_000868277.1
• Coordinates: 29699..29965 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: TsxB
• Uniprot ID: -
• Locus tag: E4U78_RS27525
• Protein ID: WP_001479502.1
• Coordinates: 29955..30197 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E4U78_RS27490	24937..25224	-	288	WP_000332251.1	helix-turn-helix transcriptional regulator	-
E4U78_RS27495	25423..25761	+	339	WP_000612441.1	helix-turn-helix domain-containing protein	-
E4U78_RS27500	26113..27117	-	1005	WP_001208090.1	replication initiation protein	-
E4U78_RS27505	28741..29064	+	324	WP_000432877.1	hypothetical protein	-
E4U78_RS27510	29104..29328	+	225	WP_000866040.1	hypothetical protein	-
E4U78_RS27515	29424..29648	+	225	WP_000749828.1	hypothetical protein	-
E4U78_RS27520	29699..29965	+	267	WP_000868277.1	hypothetical protein	Toxin
E4U78_RS27525	29955..30197	+	243	WP_001479502.1	transcriptional regulator	Antitoxin
E4U78_RS27530	30636..30965	+	330	WP_000855123.1	hypothetical protein	-
E4U78_RS27535	31023..31301	+	279	WP_000545933.1	hypothetical protein	-
E4U78_RS27540	31734..31916	+	183	WP_000833471.1	type II toxin-antitoxin system HicA family toxin	-
E4U78_RS27545	31941..32378	+	438	WP_000466317.1	type II toxin-antitoxin system HicB family antitoxin	-
E4U78_RS27550	32407..33111	-	705	WP_001279862.1	hypothetical protein	-
E4U78_RS27555	33108..33869	-	762	WP_001097938.1	conjugal transfer protein TraL	-
E4U78_RS27560	33879..34286	-	408	WP_001190621.1	hypothetical protein	-
E4U78_RS27565	34633..34968	+	336	WP_000963832.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..38515	38515

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (8-37)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10205.74 Da
• Isoelectric Point: 10.4299

>T121289 WP_000868277.1 NZ_CP038307:29699-29965 [Escherichia coli O157:H7]
MKVRKGDRQYYLNKEGNMFHLVKRVKTFSKSATLGKTKATVKTVADLVFNEKAFDTIDFSRDGLRENDKEIVSMMIQEMN
NERGKNVN

Nucleotide

• Length: 267 bp

>T121289 NZ_CP038307:29699-29965 [Escherichia coli O157:H7]
ATGAAGGTACGAAAAGGTGACAGGCAATATTATTTGAATAAAGAAGGTAATATGTTTCATCTTGTGAAACGGGTGAAGAC
CTTTTCTAAATCTGCGACTTTAGGAAAAACGAAAGCAACGGTGAAAACTGTTGCTGATCTTGTTTTTAATGAGAAGGCAT
TCGACACAATAGACTTTTCCAGAGATGGATTAAGAGAGAATGATAAAGAAATTGTTTCTATGATGATTCAGGAAATGAAC
AATGAACGAGGAAAAAATGTTAACTGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9250.74 Da
• Isoelectric Point: 8.7411

>AT121289 WP_001479502.1 NZ_CP038307:29955-30197 [Escherichia coli O157:H7]
MLTENTPENIKRLRQKIGLTQKECAEIFSMTPRTWRRKEEPVGTVSGTALTPVEFKYLQLLAGEHPEYVLCKRDKPKCSE

Nucleotide

• Length: 243 bp

>AT121289 NZ_CP038307:29955-30197 [Escherichia coli O157:H7]
ATGTTAACTGAAAACACACCAGAAAACATCAAACGTCTGCGGCAAAAAATTGGTCTGACGCAGAAAGAATGTGCCGAAAT
ATTCAGCATGACTCCGCGAACCTGGCGTCGCAAGGAAGAACCTGTCGGAACTGTAAGTGGTACAGCACTTACACCTGTGG
AGTTTAAATATCTTCAGCTTTTAGCTGGGGAACATCCAGAATACGTCCTGTGCAAAAGGGACAAGCCTAAATGTAGTGAA
TGA

Structures

Toxin

• AlphaFold DB: A0A7L6LHZ8