Detailed information of TA system
Overview
TA module
| Type | III | Classification (family/domain) | toxIN/ToxN(toxin) |
| Location | 1452..2183 | Replicon | plasmid pNE1040-2 |
| Accession | NZ_CP038306 | ||
| Organism | Escherichia coli O157:H7 strain SS NE 1040-1 | ||
Toxin (Protein)
| Gene name | toxN | Uniprot ID | - |
| Locus tag | E4U78_RS27320 | Protein ID | WP_000675353.1 |
| Coordinates | 1452..1967 (-) | Length | 172 a.a. |
Antitoxin (RNA)
| Gene name | toxI | ||
| Locus tag | - | ||
| Coordinates | 1968..2183 (-) |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| E4U78_RS27310 | 1..6042 | - | 6042 | WP_001508827.1 | hypothetical protein | - |
| E4U78_RS27315 | 630..1340 | + | 711 | WP_024177388.1 | hypothetical protein | - |
| E4U78_RS27320 | 1452..1967 | - | 516 | WP_000675353.1 | type III toxin-antitoxin system ToxN/AbiQ family toxin | Toxin |
| - | 1968..2183 | - | 216 | NuclAT_0 | - | Antitoxin |
| - | 1968..2183 | - | 216 | NuclAT_0 | - | Antitoxin |
| - | 1968..2183 | - | 216 | NuclAT_0 | - | Antitoxin |
| - | 1968..2183 | - | 216 | NuclAT_0 | - | Antitoxin |
| - | 2018..2075 | - | 58 | NuclAT_7 | - | - |
| - | 2018..2075 | - | 58 | NuclAT_7 | - | - |
| - | 2018..2075 | - | 58 | NuclAT_7 | - | - |
| - | 2018..2075 | - | 58 | NuclAT_7 | - | - |
| - | 2018..2111 | - | 94 | NuclAT_5 | - | - |
| - | 2018..2111 | - | 94 | NuclAT_5 | - | - |
| - | 2018..2111 | - | 94 | NuclAT_5 | - | - |
| - | 2018..2111 | - | 94 | NuclAT_5 | - | - |
| - | 2018..2147 | - | 130 | NuclAT_3 | - | - |
| - | 2018..2147 | - | 130 | NuclAT_3 | - | - |
| - | 2018..2147 | - | 130 | NuclAT_3 | - | - |
| - | 2018..2147 | - | 130 | NuclAT_3 | - | - |
| - | 2018..2184 | - | 167 | NuclAT_1 | - | - |
| - | 2018..2184 | - | 167 | NuclAT_1 | - | - |
| - | 2018..2184 | - | 167 | NuclAT_1 | - | - |
| - | 2018..2184 | - | 167 | NuclAT_1 | - | - |
| - | 2054..2187 | - | 134 | NuclAT_2 | - | - |
| - | 2054..2187 | - | 134 | NuclAT_2 | - | - |
| - | 2054..2187 | - | 134 | NuclAT_2 | - | - |
| - | 2054..2187 | - | 134 | NuclAT_2 | - | - |
| - | 2090..2187 | - | 98 | NuclAT_4 | - | - |
| - | 2090..2187 | - | 98 | NuclAT_4 | - | - |
| - | 2090..2187 | - | 98 | NuclAT_4 | - | - |
| - | 2090..2187 | - | 98 | NuclAT_4 | - | - |
| - | 2126..2187 | - | 62 | NuclAT_6 | - | - |
| - | 2126..2187 | - | 62 | NuclAT_6 | - | - |
| - | 2126..2187 | - | 62 | NuclAT_6 | - | - |
| - | 2126..2187 | - | 62 | NuclAT_6 | - | - |
| E4U78_RS27325 | 3251..3442 | - | 192 | WP_000165987.1 | Rop family plasmid primer RNA-binding protein | - |
| E4U78_RS27330 | 3507..3830 | + | 324 | WP_001508824.1 | MobC family plasmid mobilization relaxosome protein | - |
| E4U78_RS27335 | 3820..4530 | + | 711 | Protein_5 | relaxase/mobilization nuclease domain-containing protein | - |
| E4U78_RS27340 | 4508..5026 | + | 519 | WP_043991090.1 | MbeB family mobilization protein | - |
| E4U78_RS27345 | 5033..5266 | + | 234 | WP_029401705.1 | MbeD family mobilization/exclusion protein | - |
| E4U78_RS27350 | 5579..5860 | - | 282 | WP_024177387.1 | hypothetical protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Mobilizable plasmid | - | - | 1..6042 | 6042 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 172 a.a. Molecular weight: 19763.71 Da Isoelectric Point: 5.6847
>T121285 WP_000675353.1 NZ_CP038306:c1967-1452 [Escherichia coli O157:H7]
MKFYTISSSYIKYLKDFDDKVPNSEDPTYNNPKAFIGIVLEIEGHKYLAPLTSPKAWHANVKESSPAFFKLHENGVPDNQ
LGLINLKFMIPIIEAEVSLLDLDSMPDTPYKRMLYKQLQFIRVNEDKISEKSKLLRNLALQGRMQGTCDFAVLEEKYQHF
GKKPEDMEIDD
MKFYTISSSYIKYLKDFDDKVPNSEDPTYNNPKAFIGIVLEIEGHKYLAPLTSPKAWHANVKESSPAFFKLHENGVPDNQ
LGLINLKFMIPIIEAEVSLLDLDSMPDTPYKRMLYKQLQFIRVNEDKISEKSKLLRNLALQGRMQGTCDFAVLEEKYQHF
GKKPEDMEIDD
Download Length: 516 bp
>T121285 NZ_CP038306:c1967-1452 [Escherichia coli O157:H7]
ATGAAATTTTACACAATATCAAGCAGTTACATAAAATACCTGAAGGACTTTGATGACAAAGTTCCCAATAGCGAAGATCC
TACATACAACAATCCTAAGGCTTTCATTGGCATAGTACTAGAGATTGAAGGACATAAATATTTAGCACCTTTAACATCGC
CAAAAGCATGGCATGCTAATGTAAAAGAGTCATCACCCGCCTTCTTCAAACTTCATGAAAACGGTGTCCCTGATAATCAG
CTTGGATTGATTAATCTGAAATTTATGATTCCAATAATCGAAGCTGAAGTGTCTTTACTTGATCTGGATAGCATGCCTGA
TACGCCTTACAAAAGAATGCTATATAAACAGTTACAATTTATTCGTGTAAATGAAGATAAAATATCTGAAAAATCAAAAC
TATTAAGAAACCTTGCCTTGCAAGGCAGAATGCAAGGAACATGCGACTTTGCCGTGTTAGAAGAAAAATATCAACATTTC
GGTAAAAAACCTGAAGACATGGAAATAGACGATTAA
ATGAAATTTTACACAATATCAAGCAGTTACATAAAATACCTGAAGGACTTTGATGACAAAGTTCCCAATAGCGAAGATCC
TACATACAACAATCCTAAGGCTTTCATTGGCATAGTACTAGAGATTGAAGGACATAAATATTTAGCACCTTTAACATCGC
CAAAAGCATGGCATGCTAATGTAAAAGAGTCATCACCCGCCTTCTTCAAACTTCATGAAAACGGTGTCCCTGATAATCAG
CTTGGATTGATTAATCTGAAATTTATGATTCCAATAATCGAAGCTGAAGTGTCTTTACTTGATCTGGATAGCATGCCTGA
TACGCCTTACAAAAGAATGCTATATAAACAGTTACAATTTATTCGTGTAAATGAAGATAAAATATCTGAAAAATCAAAAC
TATTAAGAAACCTTGCCTTGCAAGGCAGAATGCAAGGAACATGCGACTTTGCCGTGTTAGAAGAAAAATATCAACATTTC
GGTAAAAAACCTGAAGACATGGAAATAGACGATTAA
Antitoxin
Download Length: 216 bp
>AT121285 NZ_CP038306:c2183-1968 [Escherichia coli O157:H7]
AGGTGATTTGCTACCTTTAAGTGCAGCTAGAAATTTAGGTGATTTGCTACCTTTAAGTGCAGCTAGAAATTTAGGTGATT
TGCTACCTTTAAGTGCAGCTAGAAATTCAGGTGATTTGCTACCTTTAAGTGCAGCTAGAAATTTAGGTCATTTACTACCT
TAAAGTAATAAAGTAAAAAAGGCGACTATACACAGTCGCCTTTTTTACGAGAAAAA
AGGTGATTTGCTACCTTTAAGTGCAGCTAGAAATTTAGGTGATTTGCTACCTTTAAGTGCAGCTAGAAATTTAGGTGATT
TGCTACCTTTAAGTGCAGCTAGAAATTCAGGTGATTTGCTACCTTTAAGTGCAGCTAGAAATTTAGGTCATTTACTACCT
TAAAGTAATAAAGTAAAAAAGGCGACTATACACAGTCGCCTTTTTTACGAGAAAAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
