Detailed information of TA system TA121276

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 3713804..3714509
• Replicon: chromosome
• Accession: NZ_CP038305
• Organism: Escherichia coli O157:H7 strain SS NE 1040-1

Toxin (Protein)

• Gene name: relE
• Uniprot ID: Q8X3N0
• Locus tag: E4U78_RS19045
• Protein ID: WP_000539519.1
• Coordinates: 3713804..3714190 (+)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: E4U78_RS19050
• Protein ID: WP_001280945.1
• Coordinates: 3714180..3714509 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E4U78_RS19025	3709808..3710434	+	627	WP_001295292.1	glutathione S-transferase GstB	-
E4U78_RS19030	3710431..3711546	-	1116	WP_000554959.1	aldose sugar dehydrogenase YliI	-
E4U78_RS19035	3711657..3712040	-	384	WP_000497137.1	biofilm formation regulator BssR	-
E4U78_RS19040	3712253..3713578	+	1326	WP_000049367.1	30S ribosomal protein S12 methylthiotransferase RimO	-
E4U78_RS19045	3713804..3714190	+	387	WP_000539519.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
E4U78_RS19050	3714180..3714509	+	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
E4U78_RS19055	3714579..3715907	-	1329	WP_000086905.1	GGDEF domain-containing protein	-
E4U78_RS19060	3715915..3718263	-	2349	WP_000950320.1	EAL domain-containing protein	-
E4U78_RS19065	3718441..3719352	-	912	WP_001236044.1	glutathione ABC transporter permease GsiD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14279.42 Da
• Isoelectric Point: 9.9296

>T121276 WP_000539519.1 NZ_CP038305:3713804-3714190 [Escherichia coli O157:H7]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGSKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T121276 NZ_CP038305:3713804-3714190 [Escherichia coli O157:H7]
ATGGGTGTGTATAAGGCAAGGCGATTCAGCCAAAGCACGAAAAAACTTGGTATTCACGATAAAGTTCTCATGGCTGCCGC
TGAAGAAGTGATGCAAGGCATATGGGAGGCTGATTTAGGTAGCGGTGTCATCAAAAAACGGTTACCCCTCCAACAAGGAA
AGAGTGGTGGAGCCAGAACAATTATCTTTTTTAAATCAGCAAATCATGTGTTTTTTTATGATGGTTGGTCCAAGAGTGGT
CTAAGCAGTAAAGGAAGCAAAGAAATCGAAGATGATGAACTGGCAGCCTACAAAAAAATGGCAAATGCATTTCTGGCCTT
TAGCAATAAGCAAATAGAGGATTTGATCGAAACTGGCTTCTTGATTGAGGTGAAAAATGAGCGATAA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT121276 WP_001280945.1 NZ_CP038305:3714180-3714509 [Escherichia coli O157:H7]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT121276 NZ_CP038305:3714180-3714509 [Escherichia coli O157:H7]
ATGAGCGATAAATTACAAATGATGCTGGAAGATGCAAAGGAACTGAATGCACTGGGCCTGATGTCTGATAGTGAGGTTGA
TGCCATTGCCGTCATGGTCAAAGCCCGTGAAGTCAGCGCCCGTATAGCCAAAGTCAAATCAATGACGGGTGAAGAGATCA
AAGCCATCCGCACCCGATACGGCATGTCACAATCCGTGCTGGCACATACAATGGGAATGTCAAAAGAAAGTGTCTCGAAA
TGGGAACGTAATGAAATAAAACCGAGCGGCCCGGCACTACGTATTTTGAATACATTGGCAATAAAAGGGCCGGAAGTTTT
TGCAAAGTAA