Detailed information of TA system TA121275

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): paaR-paaA-parE/-
• Location: 3547649..3548127
• Replicon: chromosome
• Accession: NZ_CP038305
• Organism: Escherichia coli O157:H7 strain SS NE 1040-1

Toxin (Protein)

• Gene name: parE_1
• Uniprot ID: A0A891SFN9
• Locus tag: E4U78_RS18305
• Protein ID: WP_001303876.1
• Coordinates: 3547840..3548127 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: paaA
• Uniprot ID: Q8XD67
• Locus tag: E4U78_RS18300
• Protein ID: WP_000536233.1
• Coordinates: 3547649..3547840 (+)
• Length: 64 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E4U78_RS18265	3543053..3543823	-	771	WP_000450992.1	DUF1627 domain-containing protein	-
E4U78_RS18270	3543845..3544591	-	747	WP_000788751.1	ATP-binding protein	-
E4U78_RS18275	3544598..3545689	-	1092	WP_001205823.1	hypothetical protein	-
E4U78_RS18280	3545768..3546223	+	456	WP_000273724.1	hypothetical protein	-
E4U78_RS18285	3546430..3546855	-	426	WP_000693855.1	Rha family transcriptional regulator	-
E4U78_RS18290	3546839..3547111	-	273	WP_000887453.1	helix-turn-helix domain-containing protein	-
E4U78_RS18295	3547220..3547621	+	402	WP_000986592.1	helix-turn-helix domain-containing protein	-
E4U78_RS18300	3547649..3547840	+	192	WP_000536233.1	hypothetical protein	Antitoxin
E4U78_RS18305	3547840..3548127	+	288	WP_001303876.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
E4U78_RS18310	3548405..3548560	+	156	WP_000379575.1	DUF1391 family protein	-
E4U78_RS18315	3548702..3549091	+	390	WP_000394511.1	hypothetical protein	-
E4U78_RS18320	3549278..3549463	-	186	WP_001133046.1	hypothetical protein	-
E4U78_RS18325	3550037..3550225	+	189	WP_000413705.1	cell division inhibition protein DicB	-
E4U78_RS18330	3550222..3550413	+	192	WP_001098307.1	DUF1482 family protein	-
E4U78_RS18335	3550507..3552978	+	2472	WP_000034474.1	exonuclease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 10978.84 Da
• Isoelectric Point: 10.1360

>T121275 WP_001303876.1 NZ_CP038305:3547840-3548127 [Escherichia coli O157:H7]
MLPILWLPSARDDLRQIITYIAKENPPAARRLKIRIETSVLPLSEHPYLYPPSERVSGLREIVTHPNYIILYRVAASSIE
IVSVTHSRRQFPFSI

Nucleotide

• Length: 288 bp

>T121275 NZ_CP038305:3547840-3548127 [Escherichia coli O157:H7]
ATGTTACCCATTTTATGGCTACCGTCTGCTCGCGATGATTTGCGCCAGATCATAACTTACATCGCCAAGGAGAACCCACC
GGCAGCACGTAGACTAAAAATACGCATTGAAACATCGGTATTACCTCTATCTGAGCATCCGTACTTATATCCACCAAGCG
AACGGGTTTCTGGATTGAGAGAGATCGTGACCCACCCTAACTACATAATCCTGTACAGAGTAGCTGCTTCAAGCATTGAG
ATTGTAAGCGTGACACATTCTCGGCGACAATTTCCCTTCTCTATCTGA

Antitoxin

Protein

• Length: 64 a.a.
• Molecular weight: 7025.80 Da
• Isoelectric Point: 4.0270

>AT121275 WP_000536233.1 NZ_CP038305:3547649-3547840 [Escherichia coli O157:H7]
MGTALSPIVSEFETTEQENSYNEWLRTKVTSSLADTRPAIPHDEVMAEMENLIAQIAVTNKSE

Nucleotide

• Length: 192 bp

>AT121275 NZ_CP038305:3547649-3547840 [Escherichia coli O157:H7]
ATGGGTACAGCCCTTTCTCCGATAGTTTCAGAATTCGAAACTACCGAACAAGAAAACAGTTACAACGAATGGTTGCGCAC
TAAAGTAACGTCAAGCCTTGCAGACACTCGCCCCGCAATTCCACATGACGAGGTAATGGCTGAAATGGAAAATCTTATTG
CTCAAATTGCTGTAACTAACAAGAGCGAGTAA