Detailed information of TA system TA121268

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): paaR-paaA-parE/-
• Location: 2600911..2601382
• Replicon: chromosome
• Accession: NZ_CP038305
• Organism: Escherichia coli O157:H7 strain SS NE 1040-1

Toxin (Protein)

• Gene name: parE_1
• Uniprot ID: A0A0D7C2L1
• Locus tag: E4U78_RS13190
• Protein ID: WP_001303511.1
• Coordinates: 2600911..2601189 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: paaA
• Uniprot ID: Q8XAD5
• Locus tag: E4U78_RS13195
• Protein ID: WP_001302048.1
• Coordinates: 2601191..2601382 (-)
• Length: 64 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E4U78_RS13150	2595917..2596101	-	185	Protein_2569	DUF3596 domain-containing protein	-
E4U78_RS13155	2596136..2596387	-	252	WP_000005552.1	excisionase family protein	-
E4U78_RS13160	2596460..2598931	-	2472	WP_000048458.1	exonuclease	-
E4U78_RS13165	2599024..2599215	-	192	WP_001090200.1	DUF1482 family protein	-
E4U78_RS13170	2599212..2599400	-	189	WP_000449192.1	cell division inhibition protein DicB	-
E4U78_RS13175	2599801..2599965	+	165	WP_001331716.1	hypothetical protein	-
E4U78_RS13180	2599969..2600187	-	219	WP_001171930.1	hypothetical protein	-
E4U78_RS13185	2600259..2600558	-	300	WP_001240334.1	hypothetical protein	-
E4U78_RS13190	2600911..2601189	-	279	WP_001303511.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
E4U78_RS13195	2601191..2601382	-	192	WP_001302048.1	hypothetical protein	Antitoxin
E4U78_RS13200	2601403..2601774	-	372	WP_001169686.1	hypothetical protein	-
E4U78_RS13205	2601872..2602174	+	303	WP_000172738.1	transcriptional regulator	-
E4U78_RS13210	2602171..2602596	+	426	WP_000693943.1	Rha family transcriptional regulator	-
E4U78_RS13215	2602619..2603581	+	963	WP_000095670.1	helix-turn-helix domain-containing protein	-
E4U78_RS13220	2603588..2604328	+	741	WP_000788936.1	ATP-binding protein	-
E4U78_RS13225	2604354..2605123	+	770	Protein_2584	DUF1627 domain-containing protein	-
E4U78_RS13230	2605139..2605534	+	396	WP_001118159.1	DUF977 family protein	-
E4U78_RS13235	2605591..2606175	+	585	WP_000206793.1	DUF551 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2595946..2641592	45646

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10661.25 Da
• Isoelectric Point: 5.5647

>T121268 WP_001303511.1 NZ_CP038305:c2601189-2600911 [Escherichia coli O157:H7]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Nucleotide

• Length: 279 bp

>T121268 NZ_CP038305:c2601189-2600911 [Escherichia coli O157:H7]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin

Protein

• Length: 64 a.a.
• Molecular weight: 7463.47 Da
• Isoelectric Point: 8.6828

>AT121268 WP_001302048.1 NZ_CP038305:c2601382-2601191 [Escherichia coli O157:H7]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Nucleotide

• Length: 192 bp

>AT121268 NZ_CP038305:c2601382-2601191 [Escherichia coli O157:H7]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Structures

Toxin

• PDB: 5CZF
• PDB: 5CW7
• PDB: 5CZE

Antitoxin

• PDB: 5CZF
• PDB: 3ZBE
• PDB: 5CW7
• AlphaFold DB: A0A829CTP0