Detailed information of TA system TA121253

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/DUF1778(antitoxin)
• Location: 401727..402527
• Replicon: chromosome
• Accession: NZ_CP038305
• Organism: Escherichia coli O157:H7 strain SS NE 1040-1

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: A0A891SNC1
• Locus tag: E4U78_RS01895
• Protein ID: WP_000342448.1
• Coordinates: 402000..402527 (+)
• Length: 176 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: F4NNI1
• Locus tag: E4U78_RS01890
• Protein ID: WP_001277108.1
• Coordinates: 401727..401993 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E4U78_RS01870	397385..398053	+	669	WP_000617723.1	cell division ATP-binding protein FtsE	-
E4U78_RS01875	398046..399104	+	1059	WP_001042003.1	permease-like cell division protein FtsX	-
E4U78_RS01880	399349..400203	+	855	WP_000130217.1	RNA polymerase sigma factor RpoH	-
E4U78_RS01885	400474..401577	+	1104	WP_001021996.1	branched chain amino acid ABC transporter substrate-binding protein LivJ	-
E4U78_RS01890	401727..401993	+	267	WP_001277108.1	DUF1778 domain-containing protein	Antitoxin
E4U78_RS01895	402000..402527	+	528	WP_000342448.1	GNAT family N-acetyltransferase	Toxin
E4U78_RS01900	402524..402907	-	384	WP_000778769.1	aspartate 1-decarboxylase autocleavage activator PanM	-
E4U78_RS01905	403331..404440	+	1110	WP_001301528.1	high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK	-
E4U78_RS01910	404488..405414	+	927	WP_001295111.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-
E4U78_RS01915	405411..406688	+	1278	WP_000803797.1	branched chain amino acid ABC transporter permease LivM	-
E4U78_RS01920	406685..407452	+	768	WP_000082101.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (88-150)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 176 a.a.
• Molecular weight: 19692.68 Da
• Isoelectric Point: 7.3232

>T121253 WP_000342448.1 NZ_CP038305:402000-402527 [Escherichia coli O157:H7]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNEKAHTFYKSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Nucleotide

• Length: 528 bp

>T121253 NZ_CP038305:402000-402527 [Escherichia coli O157:H7]
ATGGATGATCTGACGATAGAGATTCTGACCGATGATGCAGATTATGATCTACAGCGATTCGACTGCGGCGAGGAAGCGTT
AAATCTCTTTCTGACGACACATCTCGTTCGTCAACATCGCAACAAAATTCTGCGAGCGTATATCCTTTGTCGCAACACTC
CAGAACGTCAGGTGCTGGGATATTACACATTATGCGGCAGTTGTTTTGAACGAGCCGCATTGCCCTCGAAATCGAAACAG
AAAAAAATTCCCTACAAAAATATTCCCAGCGTTACTCTTGGGCGTCTGGCAATTGATCGTTCATTACAGGGGCAGGGATG
GGGAGCAACACTGGTTGCTCATGCCATGAAAGTCGTCTGGTCAGCCTCTTTAGCGGTAGGTATTCACGGTCTTTTTGTCG
AGGCGCTGAATGAAAAAGCCCATACGTTTTATAAATCGCTGGGCTTTATCCCTTTAGTCGGGGAAAACGAAAATGCGTTA
TTTTTCCCAACCAAATCCATTGAACTGCTTTTTACACAGAGCGATTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9910.31 Da
• Isoelectric Point: 10.0863

>AT121253 WP_001277108.1 NZ_CP038305:401727-401993 [Escherichia coli O157:H7]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Nucleotide

• Length: 267 bp

>AT121253 NZ_CP038305:401727-401993 [Escherichia coli O157:H7]
ATGTCTGCTGTTAAAAAGCAGCGTATCGATCTGCGTTTAACTGACGATGACAAAAGTATGATTGAGGAAGCGGCAGCGAT
ATCTAATCAGTCCGTCAGCCAATTTATGTTGAACAGCGCTTCGCAGCGGGCTGCGGAAGTGATTGAACAGCATCGGCGGG
TGATCCTCAATGAGGAATCCTGGACGCGGGTGATGGATGCGCTGAGTAATCCACCGTCACCAGGTGAAAAGCTAAAACGT
GCGGCAAAACGTCTTCAGGGAATGTAA

Structures

Toxin

• PDB: 6GTS

Antitoxin

• AlphaFold DB: A0A829CN24