Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 30810..31454 Replicon plasmid pTX313-2
Accession NZ_CP038303
Organism Escherichia coli O157:H7 strain SS TX 313-1

Toxin (Protein)


Gene name hicA Uniprot ID A0A7L6LII1
Locus tag E4U66_RS28410 Protein ID WP_000833471.1
Coordinates 30810..30992 (+) Length 61 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A7L6LHN8
Locus tag E4U66_RS28415 Protein ID WP_000466317.1
Coordinates 31017..31454 (+) Length 146 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E4U66_RS28385 25850..26137 - 288 WP_000332251.1 helix-turn-helix transcriptional regulator -
E4U66_RS28390 26336..26674 + 339 WP_000612441.1 helix-turn-helix domain-containing protein -
E4U66_RS28395 27026..28030 - 1005 WP_001208090.1 replication initiation protein -
E4U66_RS28400 29710..30039 + 330 WP_000855123.1 hypothetical protein -
E4U66_RS28405 30097..30375 + 279 WP_000545933.1 hypothetical protein -
E4U66_RS28410 30810..30992 + 183 WP_000833471.1 type II toxin-antitoxin system HicA family toxin Toxin
E4U66_RS28415 31017..31454 + 438 WP_000466317.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
E4U66_RS28420 31483..32187 - 705 WP_001279862.1 hypothetical protein -
E4U66_RS28425 32184..32945 - 762 WP_001097938.1 conjugal transfer protein TraL -
E4U66_RS28430 32955..33362 - 408 WP_001190621.1 hypothetical protein -
E4U66_RS28435 33708..34043 + 336 WP_000963832.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..37590 37590


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(4-60)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(7-138)


Sequences

Toxin        


Download         Length: 61 a.a.        Molecular weight: 6675.97 Da        Isoelectric Point: 11.0013

>T121248 WP_000833471.1 NZ_CP038303:30810-30992 [Escherichia coli O157:H7]
MKSADLLKELIAAGCELKRHKASSHQIWWSPITGKTFPVPHPKKDLPLGTVRSIRKMAGI

Download         Length: 183 bp

>T121248 NZ_CP038303:30810-30992 [Escherichia coli O157:H7]
ATGAAATCTGCTGACCTGTTGAAAGAACTGATAGCTGCTGGTTGTGAGTTGAAGCGGCATAAGGCGAGCAGCCACCAGAT
ATGGTGGTCGCCCATAACAGGAAAAACTTTTCCTGTGCCCCATCCGAAAAAAGACTTGCCACTGGGTACTGTCAGATCGA
TCAGAAAAATGGCGGGGATTTAA

Antitoxin


Download         Length: 146 a.a.        Molecular weight: 16227.10 Da        Isoelectric Point: 4.3135

>AT121248 WP_000466317.1 NZ_CP038303:31017-31454 [Escherichia coli O157:H7]
MFFSVGVETPKDDHTAYGITVPAFDRFDFGCVSAADTQSEIPVMAREAILAIVEEMVLSGSYSVDDIHDDGCLTYAANQD
YSHCDSWFVIDVDLSEIEGKQQRINIALPDVLIRRIDGFVRESGGVYRDRSHFLAQAARHELSYK

Download         Length: 438 bp

>AT121248 NZ_CP038303:31017-31454 [Escherichia coli O157:H7]
ATGTTTTTTTCTGTGGGTGTCGAAACACCGAAAGATGACCATACGGCATATGGTATAACCGTTCCGGCTTTTGATCGTTT
TGATTTTGGCTGTGTCTCTGCTGCCGATACTCAATCAGAAATTCCTGTGATGGCGCGAGAAGCGATATTGGCTATCGTGG
AAGAAATGGTTTTAAGTGGTTCTTATTCTGTTGATGATATTCATGATGACGGCTGTTTAACATATGCCGCAAATCAGGAC
TATAGTCACTGCGATAGCTGGTTTGTAATTGATGTTGATTTGTCTGAAATTGAAGGTAAACAACAACGTATTAATATTGC
GTTGCCTGATGTGTTGATTCGTCGTATTGATGGATTTGTCAGAGAAAGTGGCGGTGTTTACAGAGATCGTAGCCATTTTC
TTGCTCAGGCCGCGCGGCATGAACTTTCATATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7L6LII1

Antitoxin

Source ID Structure
AlphaFold DB A0A7L6LHN8

References